pCMV-SARS-CoV-2 Nsp3TM
(Plasmid
#204206)
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PurposeMammalian expression of SARS-CoV-2 Nsp3
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 204206 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV-Tag2B
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/)
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SpeciesOther
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Mutationnone, from obtained clone
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Entrez GeneORF1ab (a.k.a. GU280_gp01)
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Tag
/ Fusion Protein
- FLAGx1 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Amino acids 742-1579 of Nsp3.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-SARS-CoV-2 Nsp3TM was a gift from Allan Weissman (Addgene plasmid # 204206 ; http://n2t.net/addgene:204206 ; RRID:Addgene_204206) -
For your References section:
SARS-CoV-2 papain-like protease plays multiple roles in regulating cellular proteins in the endoplasmic reticulum. Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, Loncarek J, Tsai YC, Weissman AM. J Biol Chem. 2023 Oct 12;299(12):105346. doi: 10.1016/j.jbc.2023.105346. 10.1016/j.jbc.2023.105346 PubMed 37838170
