Skip to main content
Addgene
Showing: 1501 - 1520 of 2350 results

  1. FB399

    Plasmid
    #203635
    Purpose
    Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).
    Depositor
    Jose Marcos
    Article
    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1
    Insert
    FB367+FB396
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    203635-plasmid-map-sequence-id-410739

    Enlarge

  2. FB400

    Plasmid
    #203636
    Purpose
    Module for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).
    Depositor
    Jose Marcos
    Article
    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1
    Insert
    FB367+FB397
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    203636-plasmid-map-sequence-id-405884

    Enlarge

  4. pICH47811-pAtAct2::Cas9-nls::tSlATPase

    Plasmid
    #160221
    Purpose
    Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse
    Depositor
    Matias Kirst
    Article
    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.
    Insert
    pAtACT2::Cas9-nls::tSlATPase
    Use
    Synthetic Biology; Moclo toolkit level 1 acceptor…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    160221-plasmid-map-sequence-id-316584

    Enlarge

  6. pUASTLOTattB_vhhGFP4::T48-Baz::mCherry

    Plasmid
    #163927
    Purpose
    Expression of GrabFP-A-extracellular under control of UAS or LOP enhancer
    Depositor
    Markus Affolter
    Article
    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife
    Insert
    Fusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    163927-plasmid-map-sequence-id-323456

    Enlarge

  7. pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

    Plasmid
    #163928
    Purpose
    Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer
    Depositor
    Markus Affolter
    Article
    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife
    Insert
    Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163928-plasmid-map-sequence-id-323457

    Enlarge

  17. pEF1a-FB-dCas9-puro

    Plasmid
    #100547
    Purpose
    Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein
    Depositor
    Jian Xu
    Article
    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.
    Inserts
    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
    puromycin
    Use
    CRISPR
    Tags
    FLAG, Biotin-acceptor-site (Avitag)
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1a
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    100547-plasmid-map-sequence-id-313417

    Enlarge

  18. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid
    #102886
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102886-plasmid-map-sequence-id-194712

    Enlarge

Showing: 1501 - 1520 of 2350 results