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Plasmid#72572PurposeExpresses hormone-responsive, feedback-resistant Raf allele with an N-terminal HA tag.DepositorInsertRaf1 (RAF1 Human)
UseRetroviralTagsER* and HAExpressionMammalianMutationamino acids 306-648, serine 642 mutated to alaninePromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAuroraA-GFP-AURKA-mCherry
Plasmid#99878PurposeExpression of GFP-AURKA-mCherry in mammalian cellsDepositorInsertAURKA (AURKA Human)
UseTagsGFP and mCherryExpressionMammalianMutationPromoterAURKA minimalAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-GFPg1 (BB09)
Plasmid#139461PurposeLentiviral vector with gRNA targeting GFP; includes puromycin selectable markerDepositorInsertGFP-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-bioGEF-SUMO1nc-PURO
Plasmid#208042PurposeEnables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycinDepositorInsertSUMO1nc (SUMO1 Human)
UseLentiviralTagsLow-affinity AvitagExpressionMutationQ94P Mutation near C-terminus suppresses cleavage…PromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCF287_EFS-TiCas9-P2A-Puro
Plasmid#211646PurposeEFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.DepositorInsertTamoxifen-inducible SpyCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pScalps_Puro_mTet2 catalytic domain HxD
Plasmid#79611PurposeExpression of catalytically inactive mouse Tet2DepositorInsertTet2 (Tet2 Mouse)
UseLentiviralTagsMycExpressionMutationMutant Tet2 with H1302Y, D1304A substitutions in …PromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shGFP
Plasmid#110939PurposeTet-inducible shRNA targeting GFPDepositorInsertshGFP
UseLentiviral and RNAiTagsExpressionMutationPromoterH1/TOAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro CXCR4 siRNA-1
Plasmid#12271DepositorInsertCXCR4 siRNA (CXCR4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Teton-puro-shOCT4
Plasmid#198761Purposeconditional knockdown of OCT4DepositorInsertshOCT4 (POU5F1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro p53 shRNA 2
Plasmid#10672DepositorInsertp53 shRNA (TP53 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
BAF-S4A-tet-puro
Plasmid#199649PurposeDoxycycline-inducible BAF expressionDepositorInsertBAF (BANF1 Human)
UseLentiviralTagsExpressionMutationS4APromoterAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
BAF-S4D-tet-puro
Plasmid#199650PurposeDoxycycline-inducible BAF expressionDepositorInsertBAF (BANF1 Human)
UseLentiviralTagsExpressionMutationS4DPromoterAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQC MCS IRES Puro
Plasmid#110343Purposeγ-Retroviral transfer vector for cloning and expressing your gene of interest. IRES-driven Puromycin selection.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mTurquoise2-Puro
Plasmid#169224PurposeTargeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locusDepositorInsertDouble SapI flanked mTurquoise2-P2A-Puro
UseTargeting vector backboneTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMMP-MCS-IRES-Puro
Plasmid#36952DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2-Myc
Plasmid#161583Purposeexpression of full-length mouse Lrrk2 with C-terminal Myc tagDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationnonePromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro IRES-mCherry
Plasmid#128038PurposeRetroviral vector with IRES-mCherryDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-miR-9 sponge
Plasmid#25040DepositorInsertmiR-9 sponge
UseRetroviralTagsd2EGFPExpressionMammalianMutationPromoterAvailable sinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-JEDI-1P
Plasmid#202607PurposeGenetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)DepositorInsertJEDI-1P
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-xF2X-P2A-Puro
Plasmid#110873PurposeLentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)DepositorInsertxFNLS-2X(3.7)
UseLentiviralTags3X FLAGExpressionMutationD10A, A262T, R324L, S409I, E480K, E543D, M694I, E…PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only