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Showing: 2961 - 3000 of 149132 results

  1. pMSCV-WT-mAbl

    Plasmid
    #69027
    Purpose
    Retroviral vector encoding WT mouse c-Abl
    Depositor
    Jean Wang
    Article
    Sridevi et al PLoS One. 2013 Oct 11;8(10):e77495. doi:
    Insert
    mouse Abl-Type IV (wild type)
    Use
    Retroviral
    Tags
    FLAG tag inserted at Internal SalI site (2934 bp)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pAIDA1

    Plasmid
    #79180
    Purpose
    A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags
    Depositor
    Gen Larsson
    Article
    Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi
    Insert
    Adhesin Involved in Diffuse Adherence
    Use
    Tags
    His and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    lacUV5
    Available since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    79180-plasmid-map-sequence-id-170854

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  16. MmXYRS-4xM15

    Plasmid
    #155343
    Purpose
    Plasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.
    Depositor
    Irene Coin
    Article
    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15
    Insert
    Humanized MmXYRS/tRNAM15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/U6
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155343-plasmid-map-sequence-id-310521

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  17. pKrox24(MapERK)d1EGFP (KroxDs)

    Plasmid
    #214912
    Purpose
    for PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activation
    Depositor
    Pavel Krejčí
    Insert
    d1EGFP under the MapERK promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MapkERK promoter
    Available since
    March 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    214912-plasmid-map-sequence-id-425799

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  18. pHEA

    Plasmid
    #168297
    Purpose
    Bacterial surface display of polypeptides (nanobodies) in E. coli
    Depositor
    Luis Ángel Fernández
    Article
    Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.
    Insert
    Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
    Use
    Tags
    6xHis, E-tag, and PelB signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    LacI-Plac
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    168297-plasmid-map-sequence-id-334448

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  21. ATP1A1_G2_Dual_sgRNA

    Plasmid
    #173201
    Purpose
    Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173201-plasmid-map-sequence-id-339508

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  24. pNEU-hMbPylRS-4xU6M15

    Plasmid
    #105830
    Purpose
    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
    Depositor
    Irene Coin
    Article
    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464
    Inserts
    Humanized MbPylRS
    tRNAM15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y271A, Y349F)MbPylRS
    Promoter
    CMV and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105830-plasmid-map-sequence-id-434022

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  34. Cas9-sgRNA-A+B

    Plasmid
    #149370
    Purpose
    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites
    Depositor
    Tarek Abbas
    Article
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Inserts
    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh, PGK, and U6
    Available since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149370-plasmid-map-sequence-id-315474

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  35. TFORF2469

    Plasmid
    #142096
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    IRF8 (IRF8 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142096-plasmid-map-sequence-id-395973

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  38. TFORF2950

    Plasmid
    #144426
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MAFB (MAFB Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144426-plasmid-map-sequence-id-407318

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  40. pLM-AMA15.0 Cas9

    Plasmid
    #138944
    Purpose
    Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Article
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Expression
    Mutation
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    138944-plasmid-map-sequence-id-364383

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Showing: 2961 - 3000 of 149132 results