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Plasmid#47511Purposehuman gRNA expression vector targeting EGFPDepositorInsertgRNA-EGFP site 1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NFAT luciferase
Plasmid#17870Purpose3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissueDepositorInsert3x NFAT binding sequence (IL2 )
UseLuciferaseTagsIL2 promoter and LuciferaseExpressionMammalianMutationPromoterIL-2 promoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gαi*-BERKY3
Plasmid#158198PurposeBRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 wt
Plasmid#80876Purposemammalian expression of ALK5 WTDepositorInsertALK5 (TGFBR1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gαq*-BERKY3
Plasmid#158217PurposeBRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA flag PPAR gamma
Plasmid#8895PurposeMammalian expression of flag-tagged PPAR gammaDepositorInsertPPAR gamma (Pparg Mouse)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gα13*-BERKY3
Plasmid#158218PurposeBRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P13
Plasmid#120024PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherryDepositorInsertmCherry
UsePcr templateTagsmCherryExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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His-GFP SNAP25
Plasmid#170872PurposeMammalian expression of His-GFP SNAP25.DepositorInsertSNAP 25 (Snap25 Rat)
UseTags(His)6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-CaM
Plasmid#127393PurposeFluorescent reporter for calmodulin 1DepositorInsertCalmodulin 1 (Calm1 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterpCAGAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro
Plasmid#138152PurposeExpresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleusDepositorInserteGFP-MCS
UseLentiviralTags2PEST AND NLSExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-CmYLCV
Plasmid#205245PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertCmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
UseCRISPRTagsExpressionPlantMutationPromoterCmYLCVAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z21
Plasmid#120064PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 24xSunTagDepositorInsert24xSunTag
UsePcr templateTags24xSunTagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM6-C4-mCherry
Plasmid#66530PurposeExpresses mCherry under the control of Mutant T7 Promoter C4DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - C4Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z20
Plasmid#120063PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 10xSuntagDepositorInsert10xSuntag
UsePcr templateTags10xSuntagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV HA-MADD
Plasmid#206150PurposeCMV HA-MADDDepositorInsertMADD (MADD Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
alpha7nACHR_Nacho_mCherry
Plasmid#182939Purposealpha7 nACHR expression in mammalian cell linesDepositorInsertNACH7 (CHRNA7 Human)
UseSynthesizedTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2
Plasmid#192440PurposeEncodes mCherry-tagged wild-type TSC2 isoform 5.DepositorInsertmCherry-TSC2 (TSC2 Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CLC-EYFP
Plasmid#38011DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
UseTagsEYFP and SNAP26mExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV CEPIA3mt
Plasmid#58219PurposeGreen fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)DepositorInsertCEPIA3mt
UseTagsmycExpressionMammalianMutationGCaMP2 N263Y E282V E334D M339LPromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)
Plasmid#179005PurposeSp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)
Plasmid#179004PurposeSp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mRuby3
Plasmid#155221PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMLH1
Plasmid#129426PurposeExpression human MLH1 protein using baculovirusDepositorInserthuman MLH1 (MLH1 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMSH2
Plasmid#129423PurposeExpression human MSH2 protein using baculovirusDepositorInserthuman MSH2 (MSH2 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-SH3BP4
Plasmid#46891Purposeexpresses HA tagged SH3BP4 in mammalian cellsDepositorInsertSH3BP4 (SH3BP4 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
eGFP STAT1 WT
Plasmid#12301DepositorInsertSTAT1 (STAT1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBBK03 pCAS-Tyr-[gRNA: BsaI GFP dropout] (HygR,AmpR)
Plasmid#178987PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)
Plasmid#178988PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFZ19
Plasmid#36184DepositorTypeEmpty backboneUsePlant t-dna vectorTagsExpressionPlantMutationPromoterXVEAvailable sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
NFAT/AP-1 3x luciferase
Plasmid#11783DepositorInsertNFAT/AP-1 3x (Il2 Mouse)
UseLuciferaseTagsluciferaseExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Pur-CAG-EGFP
Plasmid#80945Purposehuman AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoterDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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7h sgRNA for 4-μHOM reporter
Plasmid#113625PurposesgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.DepositorInsert7h sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-mArg1 promoter/enhancer -31/-3810
Plasmid#34571DepositorInsertArg1 promoter/enhancer -31/-3810 (Arg1 Mouse)
UseLuciferaseTagsFirefly LuciferaseExpressionMutationPromoterAvailable sinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV CEPIA4mt
Plasmid#58220PurposeGreen fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)DepositorInsertCEPIA4mt
UseTagsmycExpressionMammalianMutationGCaMP2 N263Y E282V E334D M339L F395W D436EPromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
plk1 T210A
Plasmid#132965PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorInsertpolo-like kinase 1 T210A (PLK1 Human)
UseTagsExpressionMammalianMutationT210APromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMPRAv3:minP-GFP
Plasmid#109036PurposeMPRA v3 minimal promoter with GFP donorDepositorInsertemGFP
UseTagsExpressionMammalianMutationPromoterminPAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV10-3xFlag Skp2 WT
Plasmid#81115Purposemammalian expression of Skp2 WTDepositorInsertSKP2 (SKP2 Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Cx43-7
Plasmid#58009PurposeLocalization: Gap Junctions, Excitation: 555, Emission: 584DepositorInsertCx43
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-PIK3C2B
Plasmid#161989PurposeMammalian expression vector containing PIK3C2B tagged with GFPDepositorInsertPIK3C2B (PIK3C2B Human)
UseTagsGFPExpressionMammalianMutationsiRNA resistance to siRNA#2 with 4 silent mutatio…PromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
eGFP STAT1 Y701F
Plasmid#12302DepositorInsertSTAT1 Y701F (STAT1 Human)
UseTagseGFPExpressionMammalianMutationY701FPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
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Flag-TurboID-NES_pLX304
Plasmid#202008Purposeexpresses TurboID in the mammalian cytosol, lentiviral vectorDepositorInsertFlag-TurboID-NES
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTubulin-Electra2
Plasmid#184930PurposeTubulin labeling with blue fluorescent protein Electra2DepositorInsertTUBA1B-Electra2
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
MaLionR
Plasmid#113908PurposeRed ATP sensorDepositorInsertMaLionR
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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eGFP STAT1 S727E
Plasmid#12305DepositorInsertSTAT1 S727E (STAT1 Human)
UseTagseGFPExpressionMammalianMutationS727EPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
eGFP STAT1 S727A
Plasmid#12304DepositorInsertSTAT1 S727A (STAT1 Human)
UseTagseGFPExpressionMammalianMutationS727APromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only