Skip to main content
Addgene
Showing: 3651 - 3700 of 3976 results

  13. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Expression
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

    Enlarge

  18. pSP571

    Plasmid
    #139498
    Purpose
    CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA
    Depositor
    Serge Pelet
    Article
    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:
    Insert
    pGPD Cas9 / sgRNA (STL162)
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    pGPD
    Available since
    June 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139498-plasmid-map-sequence-id-275585

    Enlarge

  40. pSP569

    Plasmid
    #139499
    Purpose
    Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae
    Depositor
    Serge Pelet
    Article
    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:
    Insert
    pSTL1 24xPP7sl STL1 100 - 600
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    pSTL1
    Available since
    June 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139499-plasmid-map-sequence-id-292017

    Enlarge

  45. pUC57-mitoTPNOX

    Plasmid
    #87854
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    mitoTPNOX
    Use
    Tags
    C-terminal FLAG tag with a linker and mitochondri…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    87854-plasmid-map-sequence-id-175127

    Enlarge

  46. pCMV6-AC EJ7-GFP

    Plasmid
    #113617
    Purpose
    Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP
    Depositor
    Jeremy Stark
    Article
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Insert
    EJ7-GFP variant of EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    113617-plasmid-map-sequence-id-218254

    Enlarge

  49. pCMV6-AC 4-μHOM

    Plasmid
    #113619
    Purpose
    Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP
    Depositor
    Jeremy Stark
    Article
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Insert
    4-μHOM variant of EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    113619-plasmid-map-sequence-id-218256

    Enlarge

Showing: 3651 - 3700 of 3976 results