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Plasmid#78579PurposeExpression flag-USP19 1-1290 in mammalian cellsDepositorInsertflag-USP19 1-1290 (USP19 Human)
UseTagsflagExpressionMammalianMutationmutate 1291 residue to stop condonPromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-TWIST2
Plasmid#197935PurposeGateway entry vector for an inducible 3xFLAG-tagged TWIST2DepositorInsertTWIST2 (TWIST2 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICE-FLAG-NAT10-siR-G641E
Plasmid#59366PurposePlasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertNAT10 NM_024662 (NAT10 Human)
UseTagsFLAGExpressionMammalianMutationG641E and silent mutations to render the cDNA res…PromoterCMV-tetAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
STAgR_Neo
Plasmid#102992PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsTagsExpressionMutationPromoterU6Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMMB67EH-Spy
Plasmid#90102PurposeIPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.DepositorInsertYcgR (ycgR )
UseBroad-host rangeTagsExpressionBacterialMutationPromoterPtacAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
PGEX-NEDD4
Plasmid#45043DepositorInsertNEDD4-1 (NEDD4 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
Rtn3LΔ6LIR-mNeonGreen
Plasmid#169036PurposeExpress human Rtn3L with 6x LC3-interacting regions mutated in mammalian cellsDepositorInsertRtn3L (RTN3 Human)
UseTagsmNeonGreenExpressionMammalianMutationChanged following LC3-interacting regions of Rtn3…PromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK172-α-syn-TEV-GFP
Plasmid#166671PurposeBacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studiesDepositorInsertmouse synuclein (from V. Lee) (Snca Mouse)
UseTagsGFPExpressionBacterialMutationadded a TEV cleavage site sequence between synucl…PromoterCMVAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-Dnmt3a_IM
Plasmid#84478PurposeLentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)DepositorInsertdCas9-Dnmt3a_IM
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
ChR2-2A-Halo
Plasmid#22047PurposeExpression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activityDepositorInsertChR2-GFP-2A-Halo-YFP
UseTagsGFP and YFPExpressionMammalianMutationNote that there is a S147R mut in YFP. This mutat…PromoterCMVAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-pro-IL1b
Plasmid#73953PurposeExpress murine pro-interleukin-1 beta in mammalian cellsDepositorInsertinterleukin-1 beta (Il1b Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
p35S_GFP-GUS
Plasmid#167122PurposePlant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.DepositorInsertGFP-GUS
UseTagsmGFP5, GUSExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIV303
Plasmid#204978PurposeAMA1 plasmid with Aspergillus optimized Mad7 and amdS selection markerDepositorInsertsMad7
amdS
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_USP7_MATH
Plasmid#178490PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertUSP7_MATH (USP7 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR RPS27A-3xFlag_IRES-GFP
Plasmid#198390PurposeRPS27A (ubiquitin/ribosomal protein eS31 fusion) expressionDepositorInsertRPS27A (RPS27A Human)
UseLentiviralTags3xFlagExpressionMammalianMutationPromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
All_in_one_CRISPR/Cas9_LacZ
Plasmid#74293Purpose"All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screeningDepositorInsertsLacZ-alpha
Cas9
mCherry
UseCRISPRTagsHAExpressionBacterial and MammalianMutationPromoterLac promoter, SV40, and T7 promoterAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PPP1CA_Metallophos
Plasmid#109934PurposeProtein expression and purification of PPP1CA_MetallophosDepositorInsertPPP1CA_Metallophos
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Rtn4a-mNeonGreen
Plasmid#169032PurposeExpress human Rtn4aDepositorInsertRtn4a (RTN4 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN479
Plasmid#86234PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locusDepositorInsertspCas9-nickase and sgRNA against mouse rosa26 locus
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
eSrtA(4S-9) pET29b
Plasmid#75146Purposereprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificityDepositorInsertreprogrammed sortase A variant
UseTags6x HisExpressionBacterialMutationN98D S102C A104V A118T F122A K134R F144L I182V E1…PromoterT7Available sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-SIRT6-H133Y
Plasmid#102327PurposeHuman SIRT6 H133Y for mammalian expressionDepositorInsertSIRT6 (SIRT6 Human)
UseLentiviralTagsNoneExpressionMammalianMutationchanged histidine 133 to tyrosinePromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-10: bdefensin 3-SLBP-YTHDF2(1-200)
Plasmid#132546PurposeExpresses CIRTS-10 (bdefensin 3-SLBP-YTHDF2(1-200)) in mammalian cellsDepositorInsertCIRTS-10: bdefensin 3-SLBP-YTHDF2(1-200)
UseTagsFLAG tagExpressionMammalianMutationPromotercmv d0Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-p38beta
Plasmid#86833Purposemammalian expression of GFP-p38betaDepositorInsertp38beta (MAPK11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pHH0103_PLK1_Pkinase
Plasmid#109933PurposeProtein expression and purification of PLK1_PkinaseDepositorInsertPLK1_Pkinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
myc-BioID-Rab7
Plasmid#121049PurposeExpresses myc tagged BioID and human Rab7a in mammalian cellsDepositorInsertsBioID
Rab7A
UseTagsmycExpressionMammalianMutationR118G (highly promiscuous form)PromoterCMVAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki67-mCherry
Plasmid#183748PurposeExpresses EGFP-Ki67-mCherryDepositorInsertMKI67 (MKI67 Human)
UseTagsKi-67 with N-terminal EGFP tag and Ki-67 with C-…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-MinD_MinE
Plasmid#133621PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ACTG1_Actin
Plasmid#109989PurposeProtein expression and purification of ACTG1_ActinDepositorInsertACTG1_Actin
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSYNCMV tAKARα
Plasmid#119921PurposeAAV construct with tAKARα being expressed under the human synapsin promotor with WPRE enhancer.DepositorInserttAKARα
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
STAgR_tdTomato
Plasmid#102993PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsTagsExpressionMutationPromoterU6Available sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Incenp wt
Plasmid#108487Purposeexpression of vsv-INCENP wt-mCherryDepositorInsertINCENP (INCENP Human)
UseTagsVSV and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-NES
Plasmid#84635PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-KRaION2
Plasmid#177800PurposeBacterial expression of green fluorescent potassium indicator KRaION2DepositorInsertKRaION2
UseTagsExpressionBacterialMutationPromoteraraBAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
AID-mTagBFP2-loxP_myo2_neoR-loxP
Plasmid#194055PurposeDual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genomeDepositorInsertIAA17 (AXR3 Mustard Weed)
UseTagscodon optimized mTagBFP2ExpressionWormMutationPromoterNoneAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry::LANS4
Plasmid#60785PurposeControl of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear TranslocationDepositorInsertLANS4
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPI304
Plasmid#113232Purposeextrachromosomal expression vector, expression of LifeAct-mCherry/PH-pkgE-GFPDepositorInsertLifeAct & pkgE-PH
UseDictyostelium discoideum expression vector, extra…TagsmCherry & GFPExpressionMutationPromoteract15Available sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARα
Plasmid#119913PurposeMammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARα
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-Vif
Plasmid#46313DepositorInsertsp19
Vif DNA-1
linker
Vif DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only