pEN_TT 3xFLAG-TWIST2
(Plasmid
#197935)
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PurposeGateway entry vector for an inducible 3xFLAG-tagged TWIST2
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197935 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEN_TTmiRC2
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Backbone manufacturerAddgene #2752
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Vector typeGateway entry vector
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTWIST2
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Alt nameAMS
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Alt nameBBRSAY
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SpeciesH. sapiens (human)
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Entrez GeneTWIST2 (a.k.a. AMS, BBRSAY, DERMO1, FFDD3, SETLSS, bHLHa39)
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site MfeI (not destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEN_TT 3xFLAG-TWIST2 was a gift from Kevin Janes (Addgene plasmid # 197935 ; http://n2t.net/addgene:197935 ; RRID:Addgene_197935) -
For your References section:
Kinetic networks identify TWIST2 as a key regulatory node in adipogenesis. Dutta AB, Lank DS, Przanowska RK, Przanowski P, Wang L, Nguyen B, Walavalkar NM, Duarte FM, Guertin MJ. Genome Res. 2023 Feb 21;33(3):314-31. doi: 10.1101/gr.277559.122. 10.1101/gr.277559.122 PubMed 36810156
