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Showing: 2921 - 2960 of 149132 results

  1. pAN7.1

    Plasmid
    #168129
    Purpose
    Expresses hygR from the gdpA promoter with a trpC terminator. Typically used in Aspergillus species as a selection marker for resistance to hygromycin.
    Depositor
    Michael Bromley
    Article
    Zhao et al Curr Protoc Microbiol. 2019 Sep;54(1):e8
    Insert
    HygR gene
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    gdpA
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    168129-plasmid-map-sequence-id-330738

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  11. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Expression
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50586-plasmid-map-sequence-id-258465

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  24. Cas9-sgRNA-A

    Plasmid
    #149369
    Purpose
    Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter
    Depositor
    Tarek Abbas
    Article
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Inserts
    Cas9
    sgRNA-A
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh, PGK, and U6
    Available since
    Nov. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    149369-plasmid-map-sequence-id-313973

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  27. pcDNA-HDAC4-FLAG

    Plasmid
    #30485
    Depositor
    Tso-Pang Yao
    Insert
    HDAC4 (HDAC4 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    30485-plasmid-map-sequence-id-239329

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  33. pCAMBgfp

    Plasmid
    #206130
    Purpose
    This binary vector has been generated for introduction of SGFP in filamentous fungi using ATMT. Contains the hygromycin resistance gene and the SGFP gene.
    Depositor
    Anne Osbourn
    Article
    Sesma et al Nature. 2004 Sep 30;431(7008):582-6. doi
    Insert
    hygromycin resistance gene and the Promoter of ToxA+SGFP
    Use
    Binary vector for expression of sgfp in filamento…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206130-plasmid-map-sequence-id-417184

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  36. pMCRi

    Plasmid
    #207524
    Purpose
    Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
    Use
    Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207524-plasmid-map-sequence-id-409593

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  37. pC-sleeping beauty

    Plasmid
    #65487
    Purpose
    HSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.
    Depositor
    Guillaume Filion
    Insert
    sleeping beauty transposase
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    HSP
    Available since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    65487-plasmid-map-sequence-id-117817

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  38. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

    Plasmid
    #216153
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11'].
    Depositor
    Pietro Fratta
    Article
    Wilkins et al bioRxiv. 2023 Nov 15:2023.11.15.565967.
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216153-plasmid-map-sequence-id-437757

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Showing: 2921 - 2960 of 149132 results