Addgene

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pcDNA3 Flag TSC2

Plasmid

#14129

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 (TSC2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pDY1173_ADAR1p150

Plasmid

#193192

Purpose

Express full length ADAR1p150

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

ADAR1p150 (ADAR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16

Plasmid

#71728

Purpose

plasmid expressing fusion construct of Gal4 DBD and VP16 AD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD(1-147) (GAL4 Budding Yeast)

Use

Tags

VP16 AD (413-490)

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-dCas9-BFP-KRAB

Plasmid

#46911

Purpose

Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

Depositor

Jonathan Weissman, Stanley Qi

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-BFP-KRAB fusion

Use

CRISPR and Lentiviral

Tags

2xNLS, BFP, HA, and KRAB domain

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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psfaRb-Tn5

Plasmid

#183638

Purpose

Expresses anti-rabbit nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti rabbit secondary nanobody fused to Tn5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBPZpGAL4DBDUw

Plasmid

#26233

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

Zip-GAL4DBD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ccdB-Nanoluc

Plasmid

#87067

Purpose

Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

Depositor

Mikko Taipale

Type

Empty backbone

Use

Tags

Nanoluc luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPR

Plasmid

#42875

Purpose

A crRNA expression plasmid for targeting a specific sequence.

Depositor

Luciano Marraffini

Article

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

CRISPR-BsaI

Use

CRISPR; E.coli

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pYL156 (TRV RNA2)

Plasmid

#148969

Purpose

Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

Depositor

Savithramma Dinesh-Kumar

Article

Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

Insert

Modified TRV RNA2

Use

T-dna vector

Tags

Expression

Mutation

see comments section below

Promoter

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Drp1K38A

Plasmid

#45161

Depositor

Alexander van der Bliek, Richard Youle

Article

Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

Insert

Drp1 (DNM1L Human)

Use

Tags

HA (AYPYDVPDY)

Expression

Mammalian

Mutation

K38A

Promoter

CMV

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-ADRP

Plasmid

#87161

Purpose

Fluorescent protein localized to lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ADRP (PLIN2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-puro SV40 LT

Plasmid

#13970

Depositor

Thomas Roberts

Article

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

SV40 LT (SV40gp6 )

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EF1α-EGFP

Plasmid

#21320

Purpose

Control Lentiviral vector with strong EF1a promoter, expresses EGFP

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pTRKH2

Plasmid

#71312

Purpose

Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

Depositor

Todd Klaenhammer, Rodolphe Barrangou

Article

O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

Type

Empty backbone

Use

E. coli-gram positive bacteria shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Luciferase-IRES-Blast-WPRE

Plasmid

#108542

Purpose

Lentivirus vector expressing Luciferase with blasticidin resistance

Depositor

Javier Alcudia

Inserts

Luciferase
Neomycin

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1apha and PGK

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINE GFP nanobody:Halo:His6

Plasmid

#111090

Purpose

Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

Depositor

Lennart Wirthmueller

Article

Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

Insert

GFP Nanobody

Use

Tags

Halo and His6

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

T7 promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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LeGO-G2

Plasmid

#25917

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pACRISPR

Plasmid

#113348

Purpose

A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-hM4D(Gi)-mCherry

Plasmid

#50479

Purpose

Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter

Depositor

Bryan Roth

Has Service

AAV5

Insert

hM4D(Gi)-mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

See supplemental documents for DREADD mutations

Promoter

GFAP

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV-beta-catenin-IRES-GFP

Plasmid

#14717

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

beta-catenin (Ctnnb1 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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