Addgene

Showing: 1441 - 1460 of 1977 results

Results per page:

Sort results by:

pcDNA3-HA p21 K163R

Plasmid

#78787

Purpose

To overexpress p21 K163R in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K163R_mutation

Promoter

CMV

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA p21 K161R

Plasmid

#78788

Purpose

To overexpress p21 K161R in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K161R_mutation

Promoter

CMV

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NTRK1 gRNA (BRDN0001148112)

Plasmid

#75965

Purpose

3rd generation lentiviral gRNA plasmid targeting human NTRK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NTRK1 (NTRK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PKDCC gRNA (BRDN0001144772)

Plasmid

#75807

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKDCC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKDCC (PKDCC Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PKDCC gRNA (BRDN0001144824)

Plasmid

#75808

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKDCC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKDCC (PKDCC Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PKDCC gRNA (BRDN0001145657)

Plasmid

#75809

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKDCC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKDCC (PKDCC Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PKDCC gRNA (BRDN0001148026)

Plasmid

#75810

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKDCC

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKDCC (PKDCC Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS3-MT-BX-6MYC p21 1-89

Plasmid

#78784

Purpose

To overexpress p21 1-89 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Use

Tags

6xMYC

Expression

Mammalian

Mutation

Jan-89

Promoter

CMV IE94

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pmCherry-2xFYVE

Plasmid

#140050

Purpose

Expresses mCherry-tagged HRS 2xFYVE in mammalian cells

Depositor

Harald Stenmark

Article

Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:

Insert

tandem HRS FYVE domain (Hgs Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Promoter

CMV

Available since

May 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastbac-Dual-StrepII-BARD1

Plasmid

#137166

Purpose

Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

BARD1 (BARD1 Human)

Use

Tags

StrepII

Expression

Insect

Mutation

fully synthetic, codon optimized for insect expre…

Promoter

p10

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

Plasmid

#207878

Purpose

AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

Depositor

Yeh-Hsing Lao

Article

Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Type

Empty backbone

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS2280

Plasmid

#111901

Purpose

ssAAV genome with Ple345 (NEFL MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple345 (NEFL Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple345 (NEFL MiniPromoter)

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS2241

Plasmid

#111896

Purpose

ssAAV genome with Ple320 (POU4F1 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple320 (POU4F1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple320 (POU4F1 MiniPromoter)

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS2242

Plasmid

#111897

Purpose

ssAAV genome with Ple321 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple321 (TUBB3 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple321 (TUBB3 MiniPromoter)

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTT3-SP-6xHis-NTRK1(FL)-FLAG

Plasmid

#157620

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NTRK1 (NTRK1 Human)

Use

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDragon-p21

Plasmid

#155016

Purpose

For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to the cell cycle suppressor p21 in the presence of Cre and (r)tTA activity

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Insert

Cdkn1a (Cdkn1a Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF1108

Plasmid

#143254

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CREBBP (CREBBP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-1(nsp8-nsp7)(nsp12)

Plasmid

#165451

Purpose

Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

Depositor

Marc Delarue

Article

Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

Inserts

nsp12
nsp8
nsp7

Use

Tags

14Histidine-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS654 All-in-One AAV-sgRNA-hNmeCas9

Plasmid

#112139

Purpose

Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.

Depositor

Erik Sontheimer

Article

Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:

Inserts

sgRNA scaffold
Human-codon-optimized NmeCas9

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U1a and U6

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF1109

Plasmid

#143466

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CREBBP (CREBBP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge