pEMS2242
(Plasmid
#111897)
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PurposessAAV genome with Ple321 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111897 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEMS2131
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Backbone manufacturerElizabeth Simpson (Addgene plasmid # 111895)
- Backbone size w/o insert (bp) 4999
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePle321
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SpeciesH. sapiens (human)
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Insert Size (bp)1829
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Entrez GeneTUBB3 (a.k.a. CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4)
- Promoter Ple321 (TUBB3 MiniPromoter)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-rev
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter. Please note - an 11 bp insertion, which introduces a StuI site upstream of 5' ITR, was found during Addgene's Quality Control process.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMS2242 was a gift from Elizabeth Simpson (Addgene plasmid # 111897 ; http://n2t.net/addgene:111897 ; RRID:Addgene_111897) -
For your References section:
New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW. Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118. 10.1089/hum.2018.118 PubMed 30062914