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Plasmid#155117PurposeMiddle entry vector containing QF2wDepositorInsertQF2w
UseGateway middle entry vectorTagsExpressionMutationPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC0.029
Plasmid#119626PurposeLevel 0 Part. CDSDepositorInsertAbREry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP2
Plasmid#42308DepositorInsertVAMP 2
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCW-LIC-N8C_Fab3b
Plasmid#203685PurposeExpresses N8C_Fab3bDepositorInsertN8C_Fab3b
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Rho*-BERKY3
Plasmid#158220PurposeBRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
mc2 mNeon hPGK
Plasmid#206230PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
mc2 puro
Plasmid#206215PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-TPNOX
Plasmid#87853PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertTPNOX
UseTagsC-terminal FLAG tag with a linkerExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gαi*-BERKY3
Plasmid#158198PurposeBRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gβγ-BERKY3
Plasmid#158219PurposeBRET biosensor for detection of free Gβγ in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-GRK3ct
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2
Plasmid#14129DepositorInsertTSC2 (TSC2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gαq*-BERKY3
Plasmid#158217PurposeBRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gα13*-BERKY3
Plasmid#158218PurposeBRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
7b sgRNA for EJ7-GFP reporter
Plasmid#113624PurposesgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporterDepositorInsert7b sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
7a sgRNA for EJ7-GFP and 4-μHOM reporters
Plasmid#113620PurposesgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reportersDepositorInsert7a sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL-Vinculin_TurboID_Venus
Plasmid#200212PurposeLentivirus vector expressing vinculin with co-localization labeler TurboID and fluorescence reporter VenusDepositorInsertVinculin_TurboID_Venus
UseLentiviralTagsExpressionMutationPromoterT7Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
modified pcAT7-Glo1
Plasmid#160996PurposeSplicing reporter backboneDepositorInserthemoglobin subunit beta (HBB Human)
UseTagsExpressionMammalianMutationRepeated intron 1 with 40 nucelotide deletionPromoterCAGAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-i1 ONE-GO
Plasmid#204333PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-i1 ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-IRESWT-Gbetagamma ONE-GO
Plasmid#204339PurposeGPCR/G protein BRET biosensorDepositorInsertGbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-oA ONE-GO
Plasmid#204335PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-oA ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
mc2 puro EF1a
Plasmid#206221PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
7h sgRNA for 4-μHOM reporter
Plasmid#113625PurposesgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.DepositorInsert7h sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
R57C10-Flp2
Plasmid#63172PurposeR57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusionDepositorInsertyeast wild type containing a Glycine at amino acid 5 of the protein sequence
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
7i sgRNA for 4-μHOM reporter
Plasmid#113626PurposesgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomologyDepositorInsert7i sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-q ONE-GO
Plasmid#204336PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-q ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-KB1691-Nluc
Plasmid#204324PurposeGPCR/G protein BRET biosensorDepositorInsertKB1691-Nluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-i3 ONE-GO
Plasmid#204334PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-i3 ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHSB04X
Plasmid#117258PurposeGenome editing for Lactobacillus brevis ATCC367DepositorInsertCas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-cyto-KB1691-Nluc
Plasmid#204326PurposeGPCR/G protein BRET biosensorDepositorInsertcytosolic KB1691-Nluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-QFGal4-SV40pA
Plasmid#155118PurposeMiddle entry vector containing QFGal4DepositorInsertQFGal4
UseGateway middle entry vectorTagsExpressionMutationPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Myc-GRM1-IRES-CyRFP1
Plasmid#195355PurposemGluR1-IRES-cyRFP1 under the control of the pCAG promoterDepositorInsertGRM1-IRES-CyRFP1
UseTagsExpressionMammalianMutationPromoterpCAGAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
mc2 mNeon EF1a
Plasmid#206224PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-KB2123-Nluc
Plasmid#204325PurposeGPCR/G protein BRET biosensorDepositorInsertKB2123-Nluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinTS-I997A
Plasmid#111832PurposeVinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vectorDepositorInsertVinculinTS (I997A) (VCL Chicken)
UseLentiviralTagsExpressionMutationchanged vinculin isoleucine 997 to alaninePromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-YKT6
Plasmid#116946PurposeExpressing GFP-YKT6 for mammalian cellsDepositorInsertSynaptobrevin homolog YKT6 (YKT6 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
VinculinTS-A50I/I997A
Plasmid#111829PurposeVinculin tension sensor with point mutations affecting ability to bind talin and actinDepositorInsertVinculinTS (I997A)
UseTagsExpressionMammalianMutationchanged vinculin alanine 50 to isoleucine and iso…PromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF HaloTag-SYB2
Plasmid#197282PurposeEncodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseTagsExpressionMammalianMutationQ76V and F77WPromoterEF1alphaAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
BRAC/pcDNA3
Plasmid#51967Purposemammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicatorDepositorInsertBRAC
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
FL-BCAN-lentiviral-GFP
Plasmid#29704DepositorInsertBrevican (BCAN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinTS-A50I
Plasmid#111831PurposeVinculin tension sensor with point mutation affecting ability to bind talin in lentiviral expression vectorDepositorInsertVinculinTS (A50I) (VCL Chicken)
UseLentiviralTagsExpressionMutationchanged vinculin alanine 50 to isoleucinePromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Gβγ-BERKY3
Plasmid#158426PurposeBRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-HygDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-GRK3ct
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW-LIC-N8C_Fab3a
Plasmid#203689PurposeExpresses N8C_Fab3aDepositorInsertN8C_Fab3a
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Vinculin_TurboID_Venus
Plasmid#200272PurposeExpressing vinculin with co-localization labeler TurboID and fluorescence reporter VenusDepositorInsertVinculin_TurboID_Venus
UseTagsExpressionMammalianMutationPromoterT7Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
mc2 mNeon
Plasmid#206218PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Gαq*-BERKY3
Plasmid#158425PurposeBRET biosensor for detection of Gαq-GTP in lentiviral vector pLVX-IRES-HygDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
mc2 blast
Plasmid#206217PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-QFGal4-SV40pA
Plasmid#155126PurposeIn vitro transcription of QFGal4 mRNADepositorInsertQFGal4
UseExpression in zebrafishTagsExpressionMammalianMutationPromoterSP6Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SV40-Galpha-i3 ONE-GO
Plasmid#204329PurposeGPCR/G protein BRET biosensorDepositorInsertSV40 Galpha-i3 ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SV40-Galpha-q ONE-GO
Plasmid#204331PurposeGPCR/G protein BRET biosensorDepositorInsertSV40 Galpha-q ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
mc2 mCherry EF1a
Plasmid#206225PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only