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Plasmid#182932PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragmentDepositorTypeEmpty backboneUseTags3C-cleavable split GFP 11, FLAG tag, and HA signa…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only
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mVenus
Plasmid#198192PurposeSpectral correction: Expression of mVenusDepositorInsertmVenus
UseTagsExpressionMammalianMutationA206KPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c45
Plasmid#201771PurposeExpresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterTEF1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBL7010
Plasmid#136942PurposeConstitutive sfGFP expression (high), bacterial promoterDepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-GFP-N2 (FR1)
Plasmid#19364PurposeGateway entry vector for C-term eGFP fusion, frame N2DepositorInsertEnhanced Green Fluorescent Protein
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-myc-nuc (w556-1)
Plasmid#20026PurposeGateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)DepositorTypeEmpty backboneUseGateway entry vectorTagsMyc and NLSExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pME phluorin2-gpi
Plasmid#73793Purposeratiometric pHluorin2 with a gpi linkage sequence in a middle entry cloneDepositorInsertratiometric pHluorin2-gpi
UseMiddle entry cloneTagsExpressionMutationPromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b S17N
Plasmid#83156PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S17N mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationS17NPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC_sgRNA
Plasmid#68710PurposeContains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG328 td-iFAST
Plasmid#130811PurposeExpresses td-iFAST in mammalian cellsDepositorInserttd-iFAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAP-BAC
Plasmid#120229PurposeTAR cloning in yeast and low-copy maintenance of large constructs in E. coliDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-VPS35
Plasmid#166220PurposeGateway entry vector for N. furzeri VPS35DepositorArticleInsertVPS35
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad-mKalama1
Plasmid#14892PurposeBacterial expression of mKalama1 blue fluorescent proteinDepositorInsertmKalama1
UseTagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…PromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-GFP-C1 (w392-1)
Plasmid#17396PurposeGateway entry vector for N-term eGFP fusion, frame C1DepositorInsertGFP
UseEntry vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmcs
Plasmid#25755PurposeEntry vector with TRE-tight promoter followed by multiple cloning site.DepositorTypeEmpty backboneUseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G12D
Plasmid#83131PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12DPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hs.KRAS4b G12C
Plasmid#83130PurposeGateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12C mutationDepositorInsertKRAS (KRAS Human)
UseGateway entry cloneTagsExpressionMutationG12CPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR2b-mTurquoise2
Plasmid#191014PurposeGateway entry vector with the mTurquoise2 fluorescent proteinDepositorInsertmTurquoise2
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
p177bp-LacOx50-Hyg
Plasmid#210213PurposeIntegrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
UseTagsExpressionMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-TurboID-NS
Plasmid#166566PurposeMultisite Gateway middle entry clone encoding TurboID. Parton lab clone JRPDepositorInsertTurboID
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only