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pCMV enAsCas12f-HKRA-sgRNA_v7

Plasmid

#208357

Purpose

Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

Depositor

Atsushi Hoshino

Article

Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

Insert

enAsCas12f-HKRA

Use

Tags

Expression

Mammalian

Mutation

I123H/D195K/D208R/V232A

Promoter

CMV

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5B-TGFbeta receptor I K232R

Plasmid

#11763

Depositor

Jeff Wrana

Article

Wieser et al EMBO J. 1995 May 15. 14(10):2199-208.

Insert

TGFbeta receptor I (TGFBR1 Human)

Use

Tags

6xHIS

Expression

Mammalian

Mutation

K232R: kinase dead

Promoter

CMV

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV BiP-Myc-KDEL-wt

Plasmid

#27164

Purpose

Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

BiP (HSPA5 Human)

Use

Tags

KDEL and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 wt

Plasmid

#16434

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-PE5max-SF3B1-K700E

Plasmid

#200317

Purpose

Expresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.

Depositor

Lili Wang

Article

Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

Inserts

K700E-ng1+pg2
PEmax-2A-MLH1dn

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-pro-Il1b-C-Flag

Plasmid

#75131

Purpose

Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

interleukin 1 beta (Il1b Mouse)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

(please see depositor comments below)

Promoter

CMV

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-2NLS-Cas9-6XMS2

Plasmid

#166033

Purpose

Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

Depositor

Yujia Cai

Article

Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV14-3X-Flag-SARS-CoV-2 S

Plasmid

#145780

Purpose

Over expression of SARS-CoV-2 S (spike) in mammalian cell line

Depositor

Zhaohui Qian

Article

Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi:

Insert

SARS-CoV-2 S (S SARS CoV 2)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Codon optimized and C-terminal 19 amino acids del…

Promoter

CMV

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-TTP(C147R)-GG-PUF

Plasmid

#52138

Purpose

Receiving vector for PUM-HD assembly and expression in mammalian cells

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Type

Empty backbone

Use

Tags

(GGGGS)3GGGG linker, 3xFlag, PUM-HD flanking regi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRY-HF1-ABE8e

Plasmid

#185670

Purpose

Expresses SpRY-HF1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-nSpRY-HF1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

SpRY-HF1 D10A

Promoter

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD63-pHuji

Plasmid

#130902

Purpose

Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD63-pHuji (CD63 Human, synthetic)

Use

Tags

pHuji

Expression

Mammalian

Mutation

pHuji inserted between Gln-36 and Leu-37 in extra…

Promoter

CMV

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-mRuby2

Plasmid

#175294

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mRuby2

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-PE2-tagRFP-BleoR

Plasmid

#192508

Purpose

Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

Depositor

Gerald Schwank

Article

Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

PE2-P2A-tagRFP-P2A-BleoR

Use

Tags

BleoR, SV40 bpNLS, and tagRFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-flag YAP2 5SA

Plasmid

#27371

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP2 (YAP1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Serines at positions 61, 109, 127, 128, 131, 163,…

Promoter

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pMSSM110: pCMV-HA-NLS-SpuFz1-v2

Plasmid

#205277

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

C310R/D487K/T513K

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

Plasmid

#224123

Purpose

Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-mutant (Q20A_Y23A_Y24F_F35A)

Use

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Q20A_Y23A_Y24F_F35A in La(SSB)1-194

Promoter

CMV

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B EDD C2768A

Plasmid

#37189

Depositor

Charles Watts, Darren Saunders

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD C2768A (UBR5 Human)

Use

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Changed Cysteine 2768 to Alanine

Promoter

CMV

Available since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD9-pHluorin

Plasmid

#130905

Purpose

Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD9-pHluorin (CD9 Aequorea victoria, Human)

Use

Tags

pHluorin

Expression

Mammalian

Mutation

phluorin inserted between Asn-50 and Asn-51 in ex…

Promoter

CMV

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-P2A-hMLH1dn

Plasmid

#174827

Purpose

Mammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PE2-P2A-hMLH1dn

Use

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript; deletion of residues 754-…

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-ABE7.10-NG

Plasmid

#181753

Purpose

MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE7.10-NG

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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