pCMV-TTP(C147R)-GG-PUF
(Plasmid
#52138)
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Purpose(Empty Backbone) Receiving vector for PUM-HD assembly and expression in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52138 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV5
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Backbone manufacturerDr. David Russell, UTSW
- Backbone size (bp) 6316
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Vector typeMammalian Expression
- Promoter CMV
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Tags
/ Fusion Proteins
- 3xFlag (N terminal on insert)
- Tristetraprolin C147R (N terminal on insert)
- (GGGGS)3GGGG linker (N terminal on insert)
- PUM-HD flanking region 1' (N terminal on backbone)
- PUM-HD flanking region 8' (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Cloning Information
- 5′ sequencing primer CMV for
- 3′ sequencing primer ggaggggtcacagggatgc (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-TTP(C147R)-GG-PUF was a gift from Huimin Zhao (Addgene plasmid # 52138 ; http://n2t.net/addgene:52138 ; RRID:Addgene_52138) -
For your References section:
Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. Abil Z, Denard CA, Zhao H. J Biol Eng. 2014 Mar 1;8(1):7. 10.1186/1754-1611-8-7 PubMed 24581042