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pGEX6P1-DEST-Strep2
Plasmid#119753PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Strep-II tag.DepositorTypeEmpty backboneUseTagsGST and Strep-tag II fusion tagExpressionBacterialMutationPromotertac promoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
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CD44MMA-c002
Plasmid#175452PurposeProtein expression in bacterial cells (as inclusion bodies). CD44 Link module, Q23-D172. Used for crystallography.DepositorInsertCd44 (Cd44 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133843
Plasmid#152781PurposeMammalian expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pStI6
Plasmid#8616DepositorInsertRibonuclease inhibitor StI
UseTagsExpressionBacterialMutationS2G point mutation; 19 silent mutations improve p…PromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
sgRNA5_8xCTS-ECFP-pA
Plasmid#200247PurposeMammalian transfections; 8xCTS-ECFP reporter construct 5DepositorInsertsgRNA5_8xCTS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgRNA3_8xCTS-ECFP-pA
Plasmid#200245PurposeMammalian transfections; 8xCTS-ECFP reporter construct 3DepositorInsertsgRNA3_8xCTS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgRNA2_8xCTS-ECFP-pA
Plasmid#200244PurposeMammalian transfections; 8xCTS-ECFP reporter construct 2DepositorInsertsgRNA2_8xCTS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6-TM2Emp-6T_iBlue-ModFlanks
Plasmid#200236PurposeBackbone for cloning longer RNA triggers that activate iSBH-sgRNAsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDKA-c014
Plasmid#175464PurposeProtein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143. Fused to IgG Fc.DepositorInsertMDK (MDK Human)
UseTagsHRV 3C-Fc-His6 and PTPRS (Gg) SPExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
EPB41L3A-c021
Plasmid#175460PurposeProtein expression in bacterial cells. FERM domain, P107-L390. Used for crystallography (PDB:6IBE).DepositorInsertEPB41L3 (EPB41L3 Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133555
Plasmid#152982PurposeMammalian expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TapTagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133563
Plasmid#152969PurposeMammalian expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TapTagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133552
Plasmid#152954PurposeMammalian expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;S-tagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133757
Plasmid#152899PurposeMammalian expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;S-tagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133743
Plasmid#152888PurposeMammalian expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;TapTagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4134384
Plasmid#152880PurposeMammalian expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;S-tagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133814
Plasmid#152794PurposeMammalian expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133692
Plasmid#152747PurposeMammalian expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHis-003ExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits
