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mScarlet-Rab7

Plasmid

#169068

Purpose

Express human Rab7a in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab7a (RAB7A Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mScarlet-EEA1

Plasmid

#169067

Purpose

Express human EEA1 in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

EEA1 (EEA1 Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-3xFLAG-BioID2-MCS

Plasmid

#186811

Purpose

CAG promoter-driven expression of BioID2-fused protein (N terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Use

Tags

3xFLAG-BioID2

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1

Plasmid

#17791

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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pCAG-MCS-TurboID-3xFLAG

Plasmid

#186814

Purpose

CAG promoter-driven expression of BioID2-fused protein (C terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Use

Tags

TurboID-3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-3xFLAG-TurboID-MCS

Plasmid

#186813

Purpose

CAG promoter-driven expression of TurboID-fused protein (N terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Use

Tags

3xFLAG-TurboID

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Rab5DN(S34N)

Plasmid

#35141

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Dominant Negative- S34N

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-MCS-BioID2-3xFLAG

Plasmid

#186812

Purpose

CAG promoter-driven expression of BioID2-fused protein (C terminus)

Depositor

Masahito Ikawa

Article

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Use

Tags

BioID2-3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab5DN(S34N)

Plasmid

#35139

Depositor

Sergio Grinstein

Article

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Dominant Negative- S34N

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-300(HAT-)

Plasmid

#23254

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

p300 (EP300 Human)

Use

Tags

Expression

Mammalian

Mutation

H1415A E1423A Y1424A L1428S Y1430A H1434A

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only

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pCSIIhyg-RGECO1.0

Plasmid

#175813

Purpose

The lentiviral vector for R-GECO1.0, a genetically encoded red fluorescent Ca2+ sensor.

Depositor

Michiyuki Matsuda

Article

Hirota et al Endocrinology. 2022 Mar 1;163(3). pii: 6

Insert

RGECO1.0

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLSL

Plasmid

#51493

Purpose

T-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Type

Empty backbone

Use

Plant t-dna plasmid

Tags

Expression

Mutation

Promoter

virion-sense LIR and 2x35S

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-PHLPP1 N-terminal truncation

Plasmid

#22404

Purpose

Expresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cells

Depositor

Alexandra Newton

Article

Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

Insert

PHLPP1 (PHLPP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-LMO-IRES-EGFP

Plasmid

#178309

Purpose

Express the bacterial lactate 2-monooxygenase (LMO) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

Lactate 2-monooxygenase

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRKVSV Rac

Plasmid

#50535

Depositor

Kenneth Yamada

Article

Koivisto et al Exp Cell Res. 2004 Feb 1;293(1):68-80.

Insert

small GTPase (RAC1 Human)

Use

Tags

2X VSV

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV5 MYC-Smurf2 (FF29/30A) WT

Plasmid

#24604

Depositor

Jeff Wrana

Article

Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

Insert

Smurf2 (SMURF2 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

F29A, F30A

Promoter

Available since

April 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pTYF-EF1a-DLDH-IRES-EGFP

Plasmid

#178310

Purpose

Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

D-lactate dehydrogenase

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Ssh1

Plasmid

#163602

Purpose

Expresses Protein phosphatase Slingshot homolog 1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pKS1

Plasmid

#24636

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

'tesA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
pET21a - TR

Plasmid

#89621

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Thioredoxin

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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