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Plasmid#58219PurposeGreen fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)DepositorInsertCEPIA3mt
UseTagsmycExpressionMammalianMutationGCaMP2 N263Y E282V E334D M339LPromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-Tol2 transposase
Plasmid#158774PurposeExpression of Tol2 transposaseDepositorInsertTol2 tranposase
UseTagsExpressionMammalianMutationTol2 codon usage frequency well suitable for muri…PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-APEX2-C Cloning Vector
Plasmid#128146PurposeTo express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEMDepositorTypeEmpty backboneUseTagsFlag-APEX2ExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pssAAV-2-hGFAP-GeNL-WPRE-bGHp(A)
Plasmid#221455PurposeAAV vector expressing GFAP-GeNLDepositorInsertGreen enhanced Nano-lantern
UseAAVTagsExpressionMutationPromoterhGFAPAvailable sinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-Cas9
Plasmid#181752PurposeMMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.DepositorInsertMMLV-Gag-3xNES-Cas9 (Spy)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9
Plasmid#133909PurposeBacterial expression of His6-Ubc9DepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
cyto-iATPSnFR1.0
Plasmid#102550Purposecytoplasmic ATP sensor for mammalian cellsDepositorInsertiATPSnFR1.0
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Hygro (656-4)
Plasmid#17446Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, HygroDepositorHas ServiceLentiviral PrepInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
deltaNp63alpha-FLAG
Plasmid#26979DepositorInsertΔNP63alpha (TP63 Human)
UseTagsFLAGExpressionMammalianMutationdominant negative isoform of p63; aa 1–587PromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-FAM134B
Plasmid#109026Purposestable lentiviral expression of cDNADepositorInsertFAM134B (RETREG1 Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSAM_Ec
Plasmid#102939PurposeTransposon mutagenesis and Tn-seqDepositorInsertmariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP::L4440
Plasmid#11335DepositorInsertGFP
UseRNAiTagsExpressionWormMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pENN236_CRISPRcharm_Kv2
Plasmid#220839PurposeCRISPRcharm Kv2 expression in mammalian cellsDepositorInsertCRISPRcharm Kv2
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterCAGAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE-8XGTIIC-DsRED-DD
Plasmid#115798Purposelentiviral, trimethoprim (TMP) regulated YAP promoter activity reporterDepositorInsertYAP responsive promoter (YAP1 Human)
UseLentiviralTagsDsRED-DHFRExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pGP-CMV-GCaMP6m
Plasmid#40754PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertGCaMP6m
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1651-sgTelomere(F+E)
Plasmid#51024PurposeLentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeresDepositorInsertOptimized sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX-PVX
Plasmid#213040PurposePVX vector to express heterologous sequences in plantsDepositorInsertPotato virus X
UseTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35SAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAurora2
Plasmid#213134PurposeIntegrated transcriptional and translational control for stringently blue-light-activated gene expressionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
BPK1520
Plasmid#65777PurposeHuman expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNADepositorInsertSpCas9 gRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
mito-dendra2
Plasmid#55796Purposephoto-activatable mitochondrial marker for expression in mammalian cellsDepositorInsertmito-Dendra2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOS007: PercevalHR ATP/ADP sensor (cytosolic)
Plasmid#163061PurposePercevalHR ATP/ADP sensor (cytosolic)DepositorInsertPercevalHR
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ERKKTRClover
Plasmid#59138PurposeORF encoding ERK KTR mClover flanked by Gateway sequencesDepositorInsertERK Kinase Translocation Reporter (MAPK1 Mouse, Human)
UseTagsExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV CEPIA4mt
Plasmid#58220PurposeGreen fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)DepositorInsertCEPIA4mt
UseTagsmycExpressionMammalianMutationGCaMP2 N263Y E282V E334D M339L F395W D436EPromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Kl-EGFP
Plasmid#45532DepositorInsertalpha Klotho (Kl Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLX307 P2A GFP Luciferase
Plasmid#193674PurposeConstitutive lentiviral expression of LuciferaseDepositorInsertLuciferase
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_S5E2_Chrimson_tdTomato
Plasmid#219432PurposeAAV construct expressing Chrimson-tdTomato under the control of E2 regulatory elementDepositorInsertChrimson-tdTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAMD1-6
Plasmid#61566PurposeStandardized mini-Tn5 delivery plasmid, gentamicin resistantDepositorTypeEmpty backboneUseSynthetic Biology; Mini-tn5 vectorTagsExpressionMutationPromoterAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLEX-MCS UBC promoter
Plasmid#128045PurposeLentiviral vector with UBC promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-GCaMP6m-Xn
Plasmid#118976PurposeNuclear localized GCaMP-X sensor for monitoring calcium signal in nucleusDepositorInsertGCaMP6m-XN
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only