-
Plasmid#144217PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertARX (ARX Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMS82
Plasmid#68110PurposeIntegration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replicationDepositorInsertsaac3(IV)
Hyg
oriT
attP
int
UseStreptomyces integrating vectorTagsExpressionMutationFragmentPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV R-CEPIA3mt
Plasmid#140464PurposeRed fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)DepositorInsertR-CEPIA3mt
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF3549
Plasmid#145025PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRC-CMV-Rev1b
Plasmid#164443PurposeEncodes HIV-1 Rev accessory protein for pseudovirus productionDepositorInsertHIV-1 Rev
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pLX209-neo-active WRN
Plasmid#125788Purposeconstitutive, ectopic expression of human active WRNDepositorInsertmyc-active WRN (WRN Human)
UseTags5x mycExpressionMammalianMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCBASceI
Plasmid#26477PurposeI-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI siteDepositorInsertpCBASceI
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMVβ-p300-myc
Plasmid#30489PurposeMammalian expression of human p300 with myc tagDepositorInsertp300 (EP300 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-BI-hTFR1-2
Plasmid#218797PurposeRepCap for AAV productionDepositorInsertAAV Rep-Cap BI-hTFR1-2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
NPY-mCherry
Plasmid#67156PurposeExpresses NPY-mCherryDepositorInsertNPYmCherry (NPY Human)
UseTagsmCherryExpressionMammalianMutationPromotercmvAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.cTNT.PI.eGFP.WPRE.rBG
Plasmid#105543PurposeAAV expression of EGFP from cTNT promoterDepositorHas ServiceAAV9InsertcTNT-PI-EGFP
UseAAVTagsExpressionMammalianMutationPromotercTNTAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-GCaMP3
Plasmid#64853PurposeCytosol-targeted version of the Ca2+ indicator GCaMP3.DepositorInsertGCaMP3
UseTagsNuclear export signal (NES)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
E-cadherin-GFP
Plasmid#28009DepositorInsertE-cadherin (CDH1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDe-CAS9-D10A
Plasmid#61477Purposebased on pDe-CAS9, encodes Cas9 D10A nickase variantDepositorInsertCas9-D10A
UseCRISPRTagsExpressionPlantMutationcatalytic Asp10 changed to AlaPromoterPcUbi4-2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-Blast
Plasmid#83480PurposeMammalian expression of Cas9 and sgRNA scaffoldDepositorInsertBlasticidin S deaminase
UseCRISPR and LentiviralTagsExpressionMammalianMutationReplaced puromycin N-acetyltransferase on the ori…PromoterEF-1αAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
MP6
Plasmid#69669PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi CDA1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
scFLARE2
Plasmid#158700PurposeNeuronal expression of scFLARE2 componentDepositorInsertscFLARE2
UseAAVTagsHA, Flag, VP16ExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterSynAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-MCS-ASC-GFP
Plasmid#73957PurposeExpress human ASC with a C-terminal EGFP tag in mammalian cellsDepositorInsertPYD and CARD domain containing, PYCARD (PYCARD Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-SAM
Plasmid#102559PurposePiggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.DepositorInsertsMS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationD10A and N863A in Cas9 and N55K in MS2PromoterCAG and EF1AAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Myc3-Nrf2
Plasmid#21555PurposeMammalian expression of human Nrf2 with Myc tagDepositorInsertnuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
MTS-mCherry-GFP1-10-Hyg-N1
Plasmid#91957PurposeExpresses mCherry-GFP1-10 in mammalian cell mitochondriaDepositorInsertMTS-mCherry-GFP1-10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
human beta-catenin pcDNA3
Plasmid#16828PurposeMammalian expression of human beta-catenin with Flag tagDepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRPN265NB-enh-MNase
Plasmid#166035PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & RunDepositorInsertGlutathione-S-Transferase/enhancer/MNase
UseTagsGSTExpressionBacterialMutationPromoterlac promoter / tac promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
FlucWT-HA-GFP11-N1
Plasmid#91954PurposeExpresses FlucWT-HA-GFP11 in mammalian cellsDepositorInsertFlucWT-HA-GFP11
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur
Plasmid#60523PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
plenti-ATF4
Plasmid#125238PurposeATF4 expression vectorDepositorInsertATF4 (ATF4 Human)
UseLentiviralTagsFlagExpressionMutationPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
IRE1 alpha-pcDNA3.EGFP
Plasmid#13009DepositorInsertInositol Requiring Enzyme-1 (ERN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2006AvailabilityAcademic Institutions and Nonprofits only