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pCS2_IRES2_EGFP_KCNQ2 var4
Plasmid#173154PurposeFor mammalian expression of KCNQ2 and creating variantsDepositorInsertKCNQ2 (KCNQ2 Human)
UseTagsIRES2-EGFPExpressionMammalianMutationPromoterSimian CMV IE94Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2_Firefly_luc-60A
Plasmid#200395PurposeA plasmid IVT template containing a Firefly luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2_RFP-60A
Plasmid#200394PurposePlasmid IVT template encoding RFP. NOTE: In the manuscript, "mCherry" was listed as the internal control for multiple fluorescence-based experiments, but it was actually RFP.DepositorInsertRFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Jmjd3-F
Plasmid#17440PurposeExpression of Jmjd3 in mammalian cellsDepositorInsertJMJD3 (Kdm6b Mouse)
UseTags6xMyc and FlagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCS2-UTX-F
Plasmid#17438PurposeExpression of UTX in mammalian cellsDepositorInsertUTX (KDM6A Human)
UseTags6xMyc and FlagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCS2_IRES2_CyOFP1_KCNQ2 var4
Plasmid#173155PurposeFor mammalian expression of KCNQ2 and creating variantsDepositorInsertKCNQ2 (KCNQ2 Human)
UseTagsIRES2-CyOFP1ExpressionMammalianMutationPromoterSimian CMV IE94Available sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+8 Flag3HuR
Plasmid#135950PurposeExpresses HuR in mammalian cells. Can also be used to generate mRNA for zebrafish expression.DepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseTagsFlag3ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-UTY-F
Plasmid#17439PurposeExpression of UTY in mammalian cellsDepositorInsertUTY (UTY Human)
UseTags6xMyc and FlagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCS2-wt-EGFP
Plasmid#191117PurposeAn wt EGFP control for ZebrafishDepositorInsertenhanced green fluorescence protein
UseTagsHis tagExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2 hSmad1-WT
Plasmid#22989DepositorInsertSmad1 (SMAD1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCS2xVlk1-3xFlag
Plasmid#172773PurposemRNA synthesis for injection into embryosDepositorInsertVlk1 (pkdcc.1.S Frog)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MT GLI2 FL
Plasmid#17648DepositorInsertGLI2 (GLI2 Human)
UseTags6x MycExpressionMammalianMutationPromoterCMV IE94Available sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCS2_hMGFP-60A
Plasmid#200393PurposeA plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertmonster GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2_Renilla_luc-60A
Plasmid#200397PurposeA plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertRenilla luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2 hSmad1-MM
Plasmid#22990DepositorInsertSmad1 (SMAD1 Human)
UseTagsFLAGExpressionMammalianMutationS214A, S206A, S195A, S187APromoterAvailable sinceJuly 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCS2 hSmad1-GM
Plasmid#22992DepositorInsertSmad1 (SMAD1 Human)
UseTagsFLAGExpressionMammalianMutationS210A ,T202A, S191A, S198APromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCS2-XTbx5-GR
Plasmid#117248PurposemRNA synthesisDepositorInsertTbx5-GR (tbx5-b Frog)
UseXenopus expressionTagsExpressionMutationchimeric protein with human glucocorticoid recep…PromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
OSR1-pCS2+MT
Plasmid#30491DepositorInsertOSR1 (OSR1 Human)
UseTags6xMycExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2+/N-Halo
Plasmid#184417Purposevector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesisDepositorTypeEmpty backboneUseFor mrna synthesisTagsHalo-tagExpressionMutationCodon optimized for expression in Xenopus laevis …PromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only
