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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17648 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2-MT
- Backbone size w/o insert (bp) 4350
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGLI2
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SpeciesH. sapiens (human)
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Insert Size (bp)4760
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Entrez GeneGLI2 (a.k.a. CJS, HPE9, PHS2, THP1, THP2)
- Promoter CMV IE94
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Tag
/ Fusion Protein
- 6x Myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is an R250C mutation in the gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-MT GLI2 FL was a gift from Erich Roessler (Addgene plasmid # 17648 ; http://n2t.net/addgene:17648 ; RRID:Addgene_17648) -
For your References section:
A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2. Roessler E, Ermilov AN, Grange DK, Wang A, Grachtchouk M, Dlugosz AA, Muenke M. Hum Mol Genet. 2005 Aug 1. 14(15):2181-8. 10.1093/hmg/ddi222 PubMed 15994174