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PurposeExpression of UTX in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17438 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+ NLSMT
- Backbone size w/o insert (bp) 4400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUTX
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Alt nameubiquitously transcribed tetratricopeptide repeat, X chromosome
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SpeciesH. sapiens (human)
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Insert Size (bp)4200
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GenBank IDNM_021140
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Entrez GeneKDM6A (a.k.a. KABUK2, UTX, bA386N14.2)
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Tags
/ Fusion Proteins
- 6xMyc (N terminal on backbone)
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full length human UTX with a C-terminal FLAG was PCR-amplified from MGC clone BC093868 and cloned into the XhoI-XbaI sites downstream of the CMV promoter and in frame with the N-terminal 6myc tag in the pCS2+ NLSMT vector.
UTX contains K220E, V503A, N606D and T726K compared to reference sequence (NM_021140.3).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-UTX-F was a gift from Kai Ge (Addgene plasmid # 17438 ; http://n2t.net/addgene:17438 ; RRID:Addgene_17438) -
For your References section:
Identification of JmjC domain-containing UTX and JMJD3 as histone H3 lysine 27 demethylases. Hong S, Cho YW, Yu LR, Yu H, Veenstra TD, Ge K. Proc Natl Acad Sci U S A. 2007 Nov 20. 104(47):18439-44. 10.1073/pnas.0707292104 PubMed 18003914