-
Plasmid#104054PurposeAn AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoterDepositorInsert2xNLS-tdTomato
UseAAVTagsNLSExpressionMutationPromoterMBPAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCCL-c-MNDU3-X
Plasmid#81071PurposeEmpty lentiviral backbone with the MNDU3 promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterMNDU3Available sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
mIL12-N1
Plasmid#123139PurposeMammalian expression of murine cytokine IL-12 under CMV promoterDepositorInsertMurine IL12 tandem p40 p35
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIMAY
Plasmid#68939PurposeE. coli/staphylococcal temperature-sensitive plasmid for allelic exchangeDepositorTypeEmpty backboneUseStaphylococcal allelic exchange vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry
Plasmid#188766PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
AttB _EGFP
Plasmid#171597PurposeStrong EGFP fluorescence from Bxb1 landing padDepositorInsertEGFP
UseSynthetic Biology; Promoterless bxb1 dna recombin…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/CD63mScarlet
Plasmid#182972PurposeStably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.DepositorInsertCD63-mScarlet (CD63 Human)
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5154
Plasmid#175390PurposeHigh Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…TagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K27
Plasmid#22902DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK27 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCFD4-U6:1_U6:3tandemgRNAs
Plasmid#49411Purposeexpression of two gRNAs from Drosophila U6:1 and U6:3DepositorInsertsdU6-1:gRNA
dU6-3:gRNA
UseCRISPRTagsExpressionInsectMutationPromoterdU6-1 and dU6-3Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG1
Plasmid#106473PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coliDepositorInsertARG1
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQC membrane TdTomato IX
Plasmid#37351DepositorInsertmembrane TdTomato
UseTagsPalmitoylation signal*ExpressionMutationPromoterAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSXneo 270(T2AG3)
Plasmid#12403DepositorInsert270(T2AG3)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TurboID-3MYC-kanMX6 (pFB1420)
Plasmid#126049PurposeFor C-terminal tagging with TurboID in yeast including geneticin-resistant markerDepositorInsertTurboID
UsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-alpha1AR
Plasmid#106064PurposeExpression of a chimeric putative G-protein coupled receptor (Rhodopsin-alpha1AR; Opto-alpha1AR; F6; 717)DepositorInsertOpto-alpha1AR
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEB3-mScarlet3_N1
Plasmid#189770PurposeConstruct for labelling microtubule plus ends in mammalian cells with EB3-mScarlet3DepositorInsertEB3-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-EGFP BlastR
Plasmid#84383PurposeLentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsEGFPExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDuEx2-NIR-GECO1
Plasmid#113680PurposeBacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1DepositorInsertNIR-GECO1
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBA560
Plasmid#186236PurposepTet-eLbuCas13a crRNA entry vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_COCVmut_G
Plasmid#207262PurposeMammalian expression of a mutant Cocal virus G proteinDepositorInsertCOCVmut_G
UseTagsExpressionMammalianMutationK47A/Y209A/R354A; mammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHJL4
Plasmid#199379PurposeEncodes dual cistronic cytosolic jGCaMP7f and mScarlet-I optimized for C. elegans expressionDepositorInsertjGCaMP7f::SL2::mScarlet-I
UseTagsExpressionBacterialMutationno mutationsPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
MmXYRS-4xM15
Plasmid#155343PurposePlasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.DepositorInsertHumanized MmXYRS/tRNAM15
UseTagsExpressionMammalianMutationPromoterCMV/U6Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNFkB:eGFP
Plasmid#44922DepositorInsertEGFP
UseTagsExpressionMutationPromoterAvailable sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
-
pRK5-HA-Ubiquitin-K29
Plasmid#22903DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK29 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
G384A_pLenti-TetBattP(GT)-BFP-2A-iCasp9-2A-Blast_rtTA3
Plasmid#200630PurposeNegative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GT) landing padDepositorInsertiCasp9
UseLentiviralTagsExpressionMutationPromoterpTRE3GAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PRB
Plasmid#89130Purposemammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418DepositorInserthuman progesterone receptor-B (PGR Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMN-333
Plasmid#195467PurposeLentiviral Integratable Inducible synZiFTR ZF3 + ReporterDepositorInsertinverted[8X ZF3 BS - ybTATA - mCherry] - pSFFV - ZF3 - p65 - ERT2
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag p21 WT
Plasmid#16240DepositorInsertp21 (CDKN1A Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
Green Glifon4000
Plasmid#126208Purposegreen fluorescent glucose sensor for mammalian cellsDepositorInsertGreen Glifon4000
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9
Plasmid#47106PurposeExpresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cellsDepositorInsertdCas9
UseCRISPRTagsFlag and SV40 NLSExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLPCX mito roGFP2-Orp1
Plasmid#64992PurposeMammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)DepositorInsertOrp1 (HYR1 Budding Yeast)
UseRetroviralTagsroGFP2ExpressionMammalianMutationmitochondrial targeting sequencePromoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTSin EGFP
Plasmid#127695PurposePlasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequenceDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLentiCMV-Blast
Plasmid#125133PurposeLentiviral empty backbone control vector with CMV promoter and Blasticidin resistance geneDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K11
Plasmid#22901DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK11 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_Giantin_C1
Plasmid#85048PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorInsertGiantin (GOLGB1 Human)
UseTagsExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K6
Plasmid#22900DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK6 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9
Plasmid#49330PurposeExpresses sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertsCas9
dU6-sgRNA
UseCRISPRTags3xFLAG and NLSExpressionInsectMutationHuman codon optimisedPromoterActin-5c and Drosophila U6Available sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_FlagHA_USP10
Plasmid#127102DepositorInsertUSP10 (USP10 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-CUL4A
Plasmid#19951DepositorInsertCUL4A (cullin 4A) (CUL4A Human)
UseTagsmyc3ExpressionMammalianMutationPromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only