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Plasmid#102417PurposeFluorescent vector for screening enhancer elements in zebrafishDepositorInsertE1b:eGFP
UseTagsExpressionBacterialMutationPromoterZebrafish E1b minimal promoterAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestGFP_RNAseH1
Plasmid#65784Purposeexpresses GFP-tagged RNAseH1 in mammalian cellsDepositorInsertRNaseH1 (RNASEH1 Zebrafish, Human)
UseTagsGFP and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:KIT K642E
Plasmid#118849PurposeExpresses human KIT K642E mutant and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertKIT K642E (KIT Zebrafish, Human)
UseZebrafish expression, tol5TagsExpressionMutationKIT K642EPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1
Plasmid#162566PurposeUsed for the dual color labeling of cPLA2 and LaminB1 expression.DepositorUseTagsEGFP, P2A, and mKate2ExpressionMammalianMutationPromoterCMV and SV40Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCK011 p5E exorh
Plasmid#195949PurposeGateway p5E vectorDepositorInsertexorh regulatory elements
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
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p3E-U6a-polg guide
Plasmid#107598PurposeAn entry vector with U6a promoter driving polg guide RNA expressionDepositorInsertpolg guide RNA sequence
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-ubi:QFGal4-SV40pA
Plasmid#155119PurposeTol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertubi:QFGal4 (ubb Budding Yeast, Neurospora crassa, Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromoterubiquitin BAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ centrin-mKate2
Plasmid#105941Purposein vitro transcription, zf centrin N-terminally tagged with mkate2DepositorInsertCentrin
UseTagsmKate2ExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCAC-69
Plasmid#31241PurposeMitochondrial localization of EGFP, for real-time imaging of mitochondria in transgenic zebrafishDepositorInsertmitochondrial localization enhanced green fluorescent protein
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZE32 Prickle-1-GFP-pCS2+
Plasmid#17073DepositorInsertPrickle-1 (prickle1a Zebrafish)
UseZebrafish expressionTagsGFPExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
14UAS PSD95:GFP 5UAS DSRedExpress
Plasmid#74315PurposeEncodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafishDepositorInsertszebrafish PSD95
DsRedExpress
UseTagsEGFPExpressionMutationY599H and I793S (please see depositor comment bel…PromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2
Plasmid#119061PurposeFluorescent vector with zebrafish pax3a enhancer 5; Tol2 versionDepositorInsertpax3a_enh5-E1b:eGFP
UseTagsExpressionBacterialMutationPromoterE1b minimal promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestRFP_RNAseH1
Plasmid#65785Purposeexpresses RFP-tagged RNAseH1 in mammalian cellsDepositorInsertRNaseH1 (RNASEH1 Zebrafish, Human)
UseTagsRFP and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135766PurposeThis plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertWilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS
Plasmid#78251PurposeCan be used to subclone in tagged gene of interest into inducible motor neuron expression vector.DepositorInsertsUseZebrafish motor neuron inducible expressionTagsExpressionMutationPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-mitfa2.1
Plasmid#81234Purposezebrafish mitfa promoter entry vector for multisite Gateway cloningDepositorInsertmitfa promoter (mitfa Zebrafish)
UseMultisite gateway entry vectorTagsExpressionMutationPromotermitfa, 2.1kb including 5' UTRAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1- Ncad-GFP
Plasmid#35676DepositorInsertNcad (cdh2 Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3E_he1a:eYFP
Plasmid#113879Purposep3E entry vector for Three Multisite GatewayDepositorInserthe1a (he1.1 Zebrafish)
UseZebrafish danio expressionTagseYFPExpressionMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E ubb polyA
Plasmid#195977PurposeGateway p3E vectorDepositorInsertubb:polyA (ubb Zebrafish)
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only