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Showing: 121 - 140 of 18487 results

  9. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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  10. kiCAP-AAV-BI30

    Plasmid
    #183749
    Purpose
    AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with tropism for CNS endothelial cells.
    Depositor
    Ben Deverman
    Article
    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40
    Insert
    AAV9 modified with 7mer insertion between amino acids 588 and 589 of VP1
    Use
    AAV
    Tags
    Expression
    Mutation
    K449R, and NNSTRGG inserted between 588 and 589 o…
    Promoter
    p41
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    183749-plasmid-map-sequence-id-442496

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  14. CMV-Hylight

    Plasmid
    #193447
    Purpose
    A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)
    Depositor
    Richard Goodman
    Article
    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119
    Insert
    Hylight Biosensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    193447-plasmid-map-sequence-id-378450

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  16. pCMV-15F11-HA-Halo

    Plasmid
    #129592
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129592-plasmid-map-sequence-id-254268

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  18. FLX1.8NFATGFPpd2HS4

    Plasmid
    #169096
    Purpose
    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation
    Depositor
    Geoffrey Owens
    Inserts
    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    NFAT enhancer + synthetic TATA box
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169096-plasmid-map-sequence-id-338033

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  19. mCherry-EB1-8

    Plasmid
    #55035
    Purpose
    Localization: MT End Binding Protein, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    EB1 (MAPRE1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 121 - 140 of 18487 results