19,753 results

1. pBABE-hygro-hTERT

   Plasmid

   #1773

   Purpose

   Retroviral expression of hTERT. Used to create immortalized cell lines.

   Depositor

   Bob Weinberg

   Article

   Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

   Insert

   hTERT (TERT Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-G3BP1-WT

   Plasmid

   #135997

   Purpose

   WT G3BP1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   G3BP1 (G3BP1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

   Plasmid

   #198468

   Purpose

   E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

   Depositor

   Viviana Risca

   Article

   Soroczynski et al bioRxiv 2024.07.11.602973

   Insert

   10His-pG-Tn5 (E54K, L372P)
   

   Tags

   10His-pG

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   Consensus φ10 T7 Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

   Plasmid

   #188767

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Promoter

   SFFV

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. XLone-GFP

   Plasmid

   #96930

   Purpose

   Tunable and temporal expression control of GFP

   Depositor

   Xiaojun Lian

   Article

   Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

   Insert

   Green Fluorescent Protein
   

   Expression

   Mammalian

   Promoter

   TRE3GS

   Available Since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3-N90-beta-catenin

   Plasmid

   #31785

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Deletion of aa 1-90- Constitutitvely Active

   Promoter

   EF1alpha

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHCMV_Bless

   Plasmid

   #207255

   Purpose

   Mammalian expression of Baboon Endogenous Retrovirus env protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   BaboonEndogenousRetrovirus_Rless_env
   

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-ER alpha

   Plasmid

   #28230

   Purpose

   Mammalian expression of Estrogen Receptor alpha fused to EGFP

   Depositor

   Michael Mancini

   Article

   Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

   Insert

   Estrogen Receptor alpha (ESR1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiGuide-Hygro-eGFP

   Plasmid

   #99375

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a and hU6

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-Flp

    Plasmid

    #122969

    Purpose

    Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

    Depositor

    Lydia Freddolino

    Article

    Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

    Insert

    Flp recombinase
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Aug. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCMV-2E2-HA-mEGFP

    Plasmid

    #129595

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCW57.1_V5_TurboID_FLAG_Ct

    Plasmid

    #194073

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FLAG-TurboID-V5

    Expression

    Mammalian

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-ATLASsnCre

    Plasmid

    #232351

    Purpose

    Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells.

    Depositor

    Don Arnold

    Article

    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

    Has Service

    AAV5

    Insert

    ATLASsnCre
    

    Use

    AAV

    Tags

    ALFA-Tag

    Promoter

    hSyn

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBbi-RP

    Plasmid

    #60513

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCh-Drp1

    Plasmid

    #49152

    Purpose

    mammalian expression of Drp1 fused to mCherry

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    Drp1 (DNM1L Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas3cRh

    Plasmid

    #133773

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Joseph Bondy-Denomy

    Article

    Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    Cas7
    

    Expression

    Bacterial

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-CRISPR

    Plasmid

    #160047

    Purpose

    For cloning sgRNA to knockout a specific gene in piggybac plasmid

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

    Plasmid

    #188777

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57.1 Lysosomal-METRIQ

    Plasmid

    #135401

    Purpose

    Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity

    Depositor

    Eisuke Itakura

    Article

    Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

    Insert

    DNAse II alpha (DNASE2 Human)
    

    Use

    Lentiviral

    Tags

    T2A, mCherry, and sfGFP

    Expression

    Mammalian

    Mutation

    Single-nucleotide polymorphism C432T

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCherry.90 alpha

    Plasmid

    #108222

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 alpha

    Depositor

    Didier Picard

    Article

    Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

    Insert

    mCherry-Hsp90a (HSP90AA1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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