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pCAGGS-C-TEV-HAT

Plasmid

#202597

Purpose

Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Use

Tags

TEV cleavable site (ENLYFQG) and poly-histidine a…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-N-TEV-HAT

Plasmid

#202598

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Use

Tags

TEV cleavable site (ENLYFQG) and poly-histidine a…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1

Plasmid

#197421

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2

Plasmid

#197422

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3

Plasmid

#197423

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

Plasmid

#197424

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

Plasmid

#197425

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

Plasmid

#197426

Purpose

π-element knock-in into exon 5 of MAPRE1

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-Cas12m-ΔZF

Plasmid

#192276

Purpose

Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

Cas12m ΔZF

Use

CRISPR

Tags

Expression

Bacterial

Mutation

H459A, C552A

Promoter

PJ23108

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-dCas12m-ΔZF

Plasmid

#192277

Purpose

pCas-dCas12m-ΔZF (D485A, H549A, C552A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmdCas12m ΔZF

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D485A, H549A, C552A

Promoter

PJ23108

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-basic

Plasmid

#196882

Purpose

Used for preparation of double-floxed inverse ORF vectors

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Type

Empty backbone

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-1700029I15Rik-3×FLAG

Plasmid

#199689

Purpose

Expression vector of mouse 1700029I15Rik. 3XFLAG tag at C-terminus.

Depositor

Masahito Ikawa

Article

Lu et al Proc Natl Acad Sci U S A. 2023 Feb 21;12

Insert

1700029I15Rik (1700029I15Rik Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG.Myc-SARS2_3CL(C145A)

Plasmid

#200121

Purpose

Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)

Use

Tags

Myc

Expression

Mammalian

Mutation

C145A

Promoter

CAG

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-Tsks/1D4

Plasmid

#199623

Purpose

Expression vector of mouse TSKS (variant X6). 1D4 tag at C-terminus.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

Tsks (variant X6) (Tsks Mouse)

Use

Tags

1D4

Expression

Mutation

Promoter

CAG

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-mut4/1_2_VB

Plasmid

#186444

Purpose

Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Cas4 domain (K81A)

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GFP-Rab27a

Plasmid

#194985

Purpose

overexpress Rab27a

Depositor

Angelique Bordey

Insert

GFP-Rab27a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

Plasmid

#195504

Purpose

Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG, Puro, and T2A

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAS-mak10 gRNA

Plasmid

#160385

Purpose

Expresses guide RNA for targeting MAK10 in yeast

Depositor

John McCusker

Insert

mak10 gRNA

Use

Tags

Expression

Yeast

Mutation

Promoter

RNA pol III promoter (tRNA-Tyr)

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70LacZ8.1-Pcadh-LacZ

Plasmid

#191624

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-3xHAtag-SP-CLSTN2

Plasmid

#191694

Purpose

For expression coding sequencing of CLSTN2

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

signal peptide, 3xHAtag and full length coding sequence of CLSTN2

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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