Showing: 1341 - 1360 of 10428 results
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Plasmid#69457PurposeRNA-OUT A3 variant - antisense (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-OUT A3
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and J23119 (sigma 70)AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAOUTA4
Plasmid#69458PurposeRNA-OUT A4 variant - antisense (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-OUT A4
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and J23119 (sigma 70)AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4-A25C
Plasmid#69461PurposeRNA-IN S4 A25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 with A25C mutation
UseSynthetic BiologyTagsExpressionBacterialMutationA25C and mutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4-C24A
Plasmid#69462PurposeRNA-IN S4 C24A variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 with C24A mutation
UseSynthetic BiologyTagsExpressionBacterialMutationC24A and mutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4-C24AA25C
Plasmid#69463PurposeRNA-IN S4 C24AA25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 with C24A, A25C mutations
UseSynthetic BiologyTagsExpressionBacterialMutationC24A, A25C, and mutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)AvailabilityAcademic Institutions and Nonprofits only -
pJBL-anti-control
Plasmid#69464PurposeAntisense control vector for functional testing (ColE1 ori, AmpR, strong promoter/terminator)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 (sigma 70)AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-N-CretrcintG
Plasmid#69570PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1Insertkozak consensus - N-CretrcintG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-C-CreintG
Plasmid#69571PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1Insertkozak consensus - C-CreintG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pCAG-N-CretrcintG
Plasmid#69572PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cellsDepositorInsertkozak consensus - N-CretrcintG
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pCAG-C-CreintG
Plasmid#69573PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cellsDepositorInsertkozak consensus - C-CreintG
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
Beta lactamase S68TAG
Plasmid#68305PurposeBeta lactamase S68TAG geneDepositorInsertS68TAG beta lactamase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PGR-Blue
Plasmid#68374PurposeTerminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.DepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assemblyTagsExpressionBacterialMutationPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
pJH104
Plasmid#68384PurposeIntegration vector for creating Sinorhizobium meliloti Landing Pad StrainDepositorInsertattP-aadA-uidA-attP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUBC_HA_2xPP7-VP64
Plasmid#68418PurposeTransient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoterDepositorInsertPP7(deltaFG)
UseSynthetic BiologyTagsHA and VP64ExpressionMammalianMutationPP7: [delta]67_74 (non-aggregating)Promoterhuman UbCAvailabilityAcademic Institutions and Nonprofits only -
pCMV_mCherry_2A_HA_2xMS2-VP64
Plasmid#68419PurposeTransient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoterDepositorInsertMS2
UseSynthetic BiologyTagsHA and VP64ExpressionMammalianMutationMS2: V75E/A81GPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pUBC_V5_MS2-mCherry
Plasmid#68420PurposeTransient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoterDepositorInsertMS2
UseSynthetic BiologyTagsV5 and mCherryExpressionMammalianMutationMS2: V75E/A81GPromoterhuman UbCAvailabilityAcademic Institutions and Nonprofits only -
pUBC_HA_L7Ae-VP64
Plasmid#68421PurposeTransient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoterDepositorInsertL7Ae
UseSynthetic BiologyTagsHA and VP64ExpressionMammalianMutationPromoterhuman UbCAvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_TOP1
Plasmid#68423PurposeTransient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoterDepositorInsertTOP1 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[3xPP7_SL]
Plasmid#68424PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoterDepositorInsertINT construct bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[S1Apt]
Plasmid#68425PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct with S1 streptavidin aptamer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6AvailabilityAcademic Institutions and Nonprofits only
Showing: 1341 - 1360 of 10428 results