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Addgene
Showing: 1341 - 1360 of 10428 results
  1. pJBL-RNAINS4-A25C

    Plasmid
    #69461
    Purpose
    RNA-IN S4 A25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Insert
    RNA-IN S4 with A25C mutation
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    A25C and mutations for S4 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Availability
    Academic Institutions and Nonprofits only
  2. pJBL-RNAINS4-C24A

    Plasmid
    #69462
    Purpose
    RNA-IN S4 C24A variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Insert
    RNA-IN S4 with C24A mutation
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    C24A and mutations for S4 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Availability
    Academic Institutions and Nonprofits only
  3. pJBL-RNAINS4-C24AA25C

    Plasmid
    #69463
    Purpose
    RNA-IN S4 C24AA25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
    Depositor
    Insert
    RNA-IN S4 with C24A, A25C mutations
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    C24A, A25C, and mutations for S4 variant (from wt)
    Promoter
    J23119 and J23119 (sigma 70)
    Availability
    Academic Institutions and Nonprofits only
  4. pAAV-EF1a-C-CreintG

    Plasmid
    #69571
    Purpose
    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells
    Depositor
    Has Service
    AAV1
    Insert
    kozak consensus - C-CreintG
    Use
    AAV and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
  5. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Availability
    Academic Institutions and Nonprofits only
  6. pJH104

    Plasmid
    #68384
    Purpose
    Integration vector for creating Sinorhizobium meliloti Landing Pad Strain
    Depositor
    Insert
    attP-aadA-uidA-attP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pUBC_HA_2xPP7-VP64

    Plasmid
    #68418
    Purpose
    Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter
    Depositor
    Insert
    PP7(deltaFG)
    Use
    Synthetic Biology
    Tags
    HA and VP64
    Expression
    Mammalian
    Mutation
    PP7: [delta]67_74 (non-aggregating)
    Promoter
    human UbC
    Availability
    Academic Institutions and Nonprofits only
  8. pU6_(GLuc)_INT[S1Apt]

    Plasmid
    #68425
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    Insert
    INT construct with S1 streptavidin aptamer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Availability
    Academic Institutions and Nonprofits only
Showing: 1341 - 1360 of 10428 results