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Plasmid#200234PurposeBackbone for cloning iSBH-sgRNAsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only
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CNN3A-c007
Plasmid#175457PurposeProtein expression in bacterial cells. Lacking N-terminal 10 residues, G11-Y329.DepositorInsertCNN3 (CNN3 Human)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c024
Plasmid#139761PurposeProtein expression/GST pull-downDepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationG177D; contains only amino acids E41-D225PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-FLAG
Plasmid#119759PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.DepositorTypeEmpty backboneUseTagsFLAG and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-His
Plasmid#119755PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.DepositorTypeEmpty backboneUseTags10xHis and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
EPB41L3A-c000
Plasmid#175458PurposeProtein expression in bacterial cells. Entire CDS, M1-D1087.DepositorInsertEPB41L3 (EPB41L3 Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEcoFus
Plasmid#187833Purpose1st generation E. coli - Fusobacterium nucleatum shuttle vector for overexpressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
U6-TM2Emp-6T_iBlue
Plasmid#200235PurposeBackbone for cloning short RNA triggers that activate iSBH-sgRNAsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3-K230-FokI_L (wt)
Plasmid#32544DepositorInsertZFN
UseTagsHA epitopeExpressionMammalianMutationPromoterCMVAvailable sinceDec. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-Strep2
Plasmid#119758PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.DepositorTypeEmpty backboneUseTagsGST and Strep-tag II fusion tagExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-Myc
Plasmid#119757PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.DepositorTypeEmpty backboneUseTagsGST and MycExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-HA
Plasmid#119751PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.DepositorTypeEmpty backboneUseTagsGST and HAExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133370
Plasmid#152013PurposeMammalian expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;CBPExpressionMammalianMutationwtPromoterCMVAvailable sinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMT1101
Plasmid#8619DepositorInsertribonuclease Sa2, barstar
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133659
Plasmid#152704PurposeMammalian expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133446
Plasmid#152976PurposeMammalian expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMT1121
Plasmid#8620DepositorInsertribonuclease Sa, barstar
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
Landing_Pad_8xCTS-ECFP-pA
Plasmid#200242PurposeBackbone for cloning 8xCTSs (8xCRISPR-target sites)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
MDKA-c009
Plasmid#175463PurposeProtein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143DepositorInsertMDK (MDK Human)
UseTagsHis6 and PTPRS (Gg) SPExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -