-
Plasmid#160871PurposeMarmoset TargetingDepositorInsertmCerulean3
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-17
Plasmid#160717PurposeTrpC Terminator bordered by BsaI restriction sitesDepositorInserttryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-15
Plasmid#160715PurposeFor C-terminal tags with Trichoderma optimised Synthetic 3X-FLAG peptide bordered by BsaI restriction sitesDepositorInsertTrichoderma optimised Synthetic 3X-FLAG peptide
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-3
Plasmid#160703Purposetef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sitesDepositorInserttef1-alpha
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-2
Plasmid#160702Purposegpdh promoter from Trichoderma virens bordered by BsaI restriction sitesDepositorInsertgpdh
UseSynthetic BiologyTagsExpressionMutationIncorporation of BsaI restriction sitesPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSWD95D293A
Plasmid#159099PurposeCckA FRET Sensor with DITE motif mutantDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
UseTagsExpressionBacterialMutationCckA D293 mutated to A293 and V199I on mRuby3 (P…PromoterXyloseAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Gal4-RNF169UBD
Plasmid#119013PurposeMammalian expression of fusion protein of yeast Gal4 with human RNF169 Ubiquitin binding domain and BFP expressionDepositorInsertGal4 (GAL4 Budding Yeast)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR2-L3-SfoI-Venus-PBL-R1
Plasmid#141019PurposeMutisite Gateway mediated vector constructionDepositorInsertmVenus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR2-L3-SfoI-mCherry-PBL-R1
Plasmid#141022PurposeMutisite Gateway mediated vector constructionDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-DEST-R5R4-pHygTK
Plasmid#141059PurposeMutisite Gateway mediated vector constructionDepositorInsertHygTK
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-DEST-R5R4-pNeo
Plasmid#141058PurposeMutisite Gateway mediated vector constructionDepositorInsertNeomycin resistance gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-DEST-R5R4-pPuroTK
Plasmid#141060PurposeMutisite Gateway mediated vector constructionDepositorInsertPuroTK
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R2-WPRE-pA-L4
Plasmid#141063PurposeMutisite Gateway mediated vector constructionDepositorInsertWPRE
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L5-EF1_-R3
Plasmid#141037PurposeMutisite Gateway mediated vector constructionDepositorInsertEF1_ promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR2-L3-SfoI-iRFP713-PBL-R1
Plasmid#141023PurposeMutisite Gateway mediated vector constructionDepositorInsertiRFP713
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR2-L3-SfoI-IRES-EGFP-PBL-R1
Plasmid#141024PurposeMutisite Gateway mediated vector constructionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR2-L3-SfoI-tdTomato-PBL-R1
Plasmid#141021PurposeMutisite Gateway mediated vector constructionDepositorInserttdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR2-L3-SfoI-Cerulean-PBL-R1
Plasmid#141020PurposeMutisite Gateway mediated vector constructionDepositorInsertmCerulean
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Cas9n-L2
Plasmid#141039PurposeMutisite Gateway mediated vector constructionDepositorInsertCas9n
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L5-CMV-R3
Plasmid#141035PurposeMutisite Gateway mediated vector constructionDepositorInsertCMV promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR2-L3-SfoI-EGFP-PBL-R1
Plasmid#141018PurposeMutisite Gateway mediated vector constructionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEU3-NII-GLICNot-C-BIOT
Plasmid#140186PurposeUsed for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and biotinylation site, and NotI restriction siteDepositorTypeEmpty backboneUseExpression vector for a cell-free systemTagsBiotinylation and GSTExpressionMutationPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tmem98-mCherry
Plasmid#124505PurposeExpresses mCherry-tagged mouse TMEM98 in mammalian cells (C-terminal tag)DepositorInsertTmem98 (Tmem98 Mouse)
UseTagsmCherryExpressionMammalianMutationstop codon was removed to allow fusion with sGFP2…PromoterCMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE-tight2-Tmem98-FLAG
Plasmid#124506PurposeExpresses FLAG-tagged mouse TMEM98 in mammalian cells in a doxycycline-dependent manner (C-terminal tag)DepositorInsertTmem98 (Tmem98 Mouse)
UseTagsFlagExpressionMammalianMutationPromotertetO-CMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsH1R DRY->AAY-mTurquoise2
Plasmid#137786PurposeVisualization of the Histamine-1 receptor DRY mutantDepositorInsertHuman histamine receptor; DRY mutant
UseTagsExpressionMammalianMutationDRY -> AAYPromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB6-Occludin
Plasmid#136637PurposeExpression of Occludin in mammalian cellsDepositorInsertOccludin (OCLN Chicken)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsH1R DRY->AAY-mCherry
Plasmid#137788PurposeVisualization of the Histamine-1 receptor DRY mutantDepositorInsertHuman histamine receptor; DRY mutant
UseTagsExpressionMammalianMutationDRY -> AAYPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsH1R DRY->AAY-p2A-mCherry
Plasmid#137787PurposeVisualization of the Histamine-1 receptor DRY mutantDepositorInsertHuman histamine receptor; DRY mutant
UseTagsExpressionMammalianMutationDRY -> AAYPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC.061
Plasmid#119603PurposeLevel 0 part. PromoterDepositorInsertPtic
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC.060
Plasmid#119602PurposeLevel 0 part. PromoterDepositorInsertPtac
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0015
Plasmid#117551PurposeLevel 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2
Plasmid#128760PurposeExpresses PARN gRNA for CRISPRiDepositorInsertPARN sgRNA #2 (PARN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3
Plasmid#128758PurposeExpresses PAPD4 gRNA for CRISPRiDepositorInsertPAPD4 sgRNA #3 (TENT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1
Plasmid#128756PurposeExpresses PAPD4 gRNA for CRISPRiDepositorInsertPAPD4 sgRNA #1 (TENT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2
Plasmid#128757PurposeExpresses PAPD4 gRNA for CRISPRiDepositorInsertPAPD4 sgRNA #1 (TENT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.284
Plasmid#119650PurposeLevel 0 Part. Insertion siteDepositorInsert7002 NS1 DOWN Flank
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.283
Plasmid#119649PurposeLevel 0 Part. Insertion siteDepositorInsert7002 NS1 UP Flank
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.282
Plasmid#119648PurposeLevel 0 Part. Insertion siteDepositorInsert7002 NS2 DOWN Flank
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.281
Plasmid#119647PurposeLevel 0 Part. Insertion siteDepositorInsert7002 NS2 UP Flank
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.177
Plasmid#119646PurposeLevel 0 Part. Insertion siteDepositorInsert7942 NS3 Up Flank
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only