pTRE-tight2-Tmem98-FLAG
(Plasmid #124506)

• Purpose: Expresses FLAG-tagged mouse TMEM98 in mammalian cells in a doxycycline-dependent manner (C-terminal tag)
• Depositing Lab: Renee van Amerongen
• Publication: van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. doi: 10.1371/journal.pone.0227435. eCollection 2020.

Backbone

• Vector backbone: pTretight-2
• Backbone manufacturer: https://www.addgene.org/19407/
• Backbone size w/o insert (bp): 4200
• Total vector size (bp): 4900
• Modifications to backbone: an SV40-puromycin-polyA resistance cassette was inserted into the backbone to allow selection in mammalian cells
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tmem98
• Species: M. musculus (mouse)
• Insert Size (bp): 770
• Entrez Gene: Tmem98 (a.k.a. 6530411B15Rik, AI463522)
• Promoter: tetO-CMV
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SV40 polyA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/512426v1 for BioRxiv preprint