Purpose
Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA
Inserts
sSpCas9
tracrRNA
crRNA cassette
Expression
Bacterial
Mutation
BbsI-flanked lacZ cassette inserted in place of s…
Promoter
gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI
Availability
Academic Institutions and Nonprofits only