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Plasmid#154337PurposePromoter reporterDepositorInsertSV40 NLS::mScarlet_I::PEST (dpi)::-tbb-2 3'UTR
UseTagsExpressionWormMutationSilent mutations to remove piRNA sitesPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID*
Plasmid#99515PurposeC-terminal AID* degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(71-114) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTagsExpressionMutationinternal inversion of nucleotides 265-312 of the …PromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHJ4: LexA-VHH-Caffeine
Plasmid#108242PurposeLexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.DepositorInsertLexA DNA binding domain fusing with VHH-Caffeine antibody
UseSynthetic BiologyTagsExpressionMutationPromoterpLacO1Available sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKan-PRFA1-9myc-AID*(N)
Plasmid#99528PurposeN-terminal 9myc-AID* degron cassette with RFA1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-117) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycExpressionMutationPromoterRFA1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pS848∙CsR
Plasmid#199240PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Gm resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7Available sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SauriCas9-KKH-Puro
Plasmid#135966PurposeExpresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-iSpyMac
Plasmid#149553PurposeGateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMsDepositorInsertiSpyMacCas9
UseCRISPR; Gateway compatible zispymaccas9 entry clo…Tags3X FLAG, NLS and NLSExpressionPlantMutationR221K, N394K, Protospacer interacting domain form…PromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OKMS-Cas9
Plasmid#120353PurposepiggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.DepositorInsertOKMS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [10-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR-SUMO1
Plasmid#114388PurposepDONR vector with SUMO1 geneDepositorInsertSUMO1 (SUMO1 Human)
UseTagsExpressionMammalianMutationQ29H compared with NCBI reference NP_003343.1PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1 Flag ATXN1L
Plasmid#33242DepositorInsertATXN1L (ATXN1L Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJW1659
Plasmid#154314PurposeNT Donor-cassette for SapTrapDepositorInsert30aa linker::AID*::3xFLAG for NT slot
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-Lmo2
Plasmid#60666PurposePiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoterDepositorInsertLmo2 (Lmo2 Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-CNL
Plasmid#65712PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-CNL)DepositorInsert7xTcf, minCMV, 3xNLS, CNL
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJW1347
Plasmid#154310PurposeDonor-cassette for SapTrapDepositorInsert30aa Linker for CT slot
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OK+9MS-Cas9
Plasmid#120354PurposepiggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.DepositorInsertOK+9MS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [1-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-ONL
Plasmid#65713PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-ONL)DepositorInsert7xTcf, minCMV, 3xNLS, ONL
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJT112_GalL_nCDA1-xBE3
Plasmid#141318PurposeExpressing base editor nCDA1-xBE3 in yeast cellsDepositorInsertnCDA1-xBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…PromoterGalLAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC234r-ccdB/TPK2
Plasmid#176179Purposeyeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)DepositorInsertdropout cassette containing toxic genes ccdB and TPK2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1820
Plasmid#154321PurposeMulti-cassette for SapTrapDepositorInsertGFP^SEC (LoxP)^AID*::3xFLAG
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
AtS/MAR10 insulator sequence (GB3458)
Plasmid#160638PurposepEGB3a1 AtS/MAR10DepositorInsertAtS/MAR10 insulator sequence
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only