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Plasmid#139760PurposeProtein expression/GST pull-downDepositorInsertTBXT, DNA-binding domain, WT (TBXT Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationContains only amino acids E41-D225PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c022
Plasmid#139759PurposeProtein expression/crystallographyDepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
UseTagsHis6-SUMOExpressionBacterialMutationG177D; contains only amino acids E41-A214PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry
Plasmid#128744PurposeExpresses mCherry in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-MNase
Plasmid#136291PurposeExpresses Micrococcal nucleaseDepositorInsertMNase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT
Plasmid#26106PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationPromoterAvailable sinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-HA
Plasmid#119756PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.DepositorTypeEmpty backboneUseTagsGST and HAExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4
Plasmid#26103PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHis - TEVExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pOpen-RNAse A
Plasmid#165565PurposeRibonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.DepositorInsertRNAse A
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX6P1-DEST
Plasmid#119749PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.DepositorTypeEmpty backboneUseTagsGSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB
Plasmid#26107PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHis6 - Z-basic - TEVExpressionBacterialMutationPromoterAvailable sinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CTHF
Plasmid#26105PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsTEV - His6 - FlagExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c043
Plasmid#139766PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, DNA-binding domain, WT (TBXT Human)
UseTagsAvi-tag (Biotin) and His6-TEVExpressionBacterialMutationContains only amino acids E41- D225PromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHMGWA-Pa14Csy4H29A
Plasmid#41092DepositorInsertCsy4
UseTagsHis6-MBPExpressionMutationHis29Ala mmutation to abolish ribonuclease activi…PromoterT7Available sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
PRDX1A-c001
Plasmid#175473PurposeProtein expression in bacterial cells. Entire CDS, M1-K199. Can be biotinylated.DepositorInsertPRDX1 (PRDX1 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c027
Plasmid#139764PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, Full-length, G177D (TBXT Human)
UseTagsAvi-tag (Biotin) and His6-GST-TEVExpressionBacterialMutationG177D; contains only amino acids S2- M435PromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF6V5:eGFP-CAAX-2A-mCherry
Plasmid#26901DepositorInserteGFP-CAAX-PTVI-2A-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c028
Plasmid#139765PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, Full-length, WT (TBXT Human)
UseTagsAvi-tag (Biotin) and His6-GST-TEVExpressionBacterialMutationContains only amino acids S2- M435PromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c044
Plasmid#139767PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
UseTagsAvi-tag (Biotin) and His6-TEVExpressionBacterialMutationG177D; contains only amino acids E41- D225PromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCYP119
Plasmid#50771Purposeexpresses CYP 119 in e. coliDepositorInsertCYP119
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
CNN3A-c002
Plasmid#175456PurposeProtein expression in bacterial cells. Entire CDS, M1-Y329.DepositorInsertCNN3 (CNN3 Human)
UseTagsHis6-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only