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Plasmid#225756PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only
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pAV-puro-U6+27-F30-Tornado-Squash-FTL-IRE
Plasmid#216828PurposeExpresses a circular RNA containing a Squash aptamer and an IRE (FTL derived) in mammalian cells, using the Tornado expression system.DepositorInsertTornado-Squash-FTL-IRE
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-puro-U6+27-F30-Tornado-Squash-TfR-IRE
Plasmid#216829PurposeExpresses a circular RNA containing a Squash aptamer and an IRE (TfR derived) in mammalian cells, using the Tornado expression system.DepositorInsertTornado-Squash-TfR-IRE
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro CCND2-T2A-luciferase-P2A-eGFP
Plasmid#179843PurposeTunable and temporal expression control of CCND2, luciferase and nuclear eGFPDepositorInsertCCND2 (CCND2 Human)
UseLuciferase and Synthetic BiologyTagseGFP and luciferaseExpressionMammalianMutationPromoterTRE3GAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
Plasmid#217970PurposeProvides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.DepositorInsert2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-IRES-puro6 gal4DBD-HIFCAD myc tag
Plasmid#207171Purposeexpression plasmid for Gal4DBD-HIF-CAD.myc expressionDepositorInsertGal4DBD-HIF1a-CAD Myc tag linker
UseTags2xMyc tagExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
m.3047-Right Puro DdCBE-G1397-C- T1413I-GFP
Plasmid#211342PurposeExpression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selectionDepositorInsertm.3047-Right Puro DdCBE-G1397-C- T1413I-GFP
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
Plasmid#211343PurposeExpression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selectionDepositorInsertm.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
Plasmid#211344PurposeExpression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selectionDepositorInsertm.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
UseTALENTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE)
Plasmid#214878PurposeLentiviral vector encoding RfxCas13d targeting SURF1 guide arrayDepositorInserthU6-crSURF1-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7820 pHR (hU6-crPURI-EFS-PuroR-WPRE)
Plasmid#214883PurposeLentiviral vector encoding RfxCas13d targeting PURI guide arrayDepositorInserthU6-crPURI-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7578 pHR (hU6-crPROX-EFS-PuroR-WPRE)
Plasmid#214882PurposeLentiviral vector encoding RfxCas13d targeting PROX guide arrayDepositorInserthU6-crPROX-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)
Plasmid#214879PurposeLentiviral vector encoding RfxCas13d targeting SURF2 guide arrayDepositorInserthU6-crSURF2-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7854 pHR (hU6-crGLY-EFS-PuroR-WPRE)
Plasmid#214884PurposeLentiviral vector encoding RfxCas13d targeting GLY guide arrayDepositorInserthU6-crGLY-EFS-PuroR-WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/134R]
Plasmid#188173PurposeMammalian Expression Plasmid of anti-NPY2R/Neuropeptide Y receptor type 2 (Human). Derived from hybridoma L119/134DepositorInsertanti-NPY2R/Neuropeptide Y receptor type 2 (Homo sapiens) recombinant mouse monoclonal antibody (NPY2R Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro
Plasmid#211687PurposeU6-sgRNA-EFS-Cas9-P2A-PuroDepositorInsertSpyCas9 and guide RNA recipient vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 S141A (C)-C-Myc-DDK-P2A-Puro
Plasmid#211622PurposeRhno1 S141ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationS141APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 T52A (B)-C-Myc-DDK-P2A-Puro
Plasmid#211621PurposeRhno1 T52ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationT52APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 S51A (A)-C-Myc-DDK-P2A-Puro
Plasmid#211620PurposeRhno1 S51ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationS51APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 S163A (E)-C-Myc-DDK-P2A-Puro
Plasmid#211624PurposeRhno1 S163ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationS163APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 S162A (D)-C-Myc-DDK-P2A-Puro
Plasmid#211623PurposeRhno1 S162ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationS162APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 S164A (F)-C-Myc-DDK-P2A-Puro
Plasmid#211625PurposeRhno1 S164ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationS164APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 S178A (G)-C-Myc-DDK-P2A-Puro
Plasmid#211626PurposeRhno1 S178ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationS178APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR
Plasmid#211815Purposelentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishmentDepositorInsertSpCas9, tracrRNA and puromycin selection gene
UseLentiviralTagsExpressionMammalianMutationPromoterU1a promoter, U6 promoter, and hPGK promoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)
Plasmid#209086PurposeNgn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expressionDepositorUseTagsAviTagExpressionMammalianMutationhuman codon optimizedPromoterTRE4G and hSynAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
anti-NPY/Neuropeptide Y scFv [L115/13] with sortase tag
Plasmid#204426PurposeMammalian Expression of anti-Neuropeptide Y scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L115/13.DepositorInsertRecombinant mouse scFv targeting NPY/Neuropeptide Y (Homo sapiens)
UseTags6xHis tag and Sortase tagExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CCDB-N136-RsDddA-B-UGI-PURO
Plasmid#205464PurposeA backbone containing a RsDddA fragment B for constructing mitochondria-targeted RsDdCBE expression plasmidDepositorInsertMTS; N136; ccdb; RsDddA-B; UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CCDB-N136-RsDddA-D-UGI-PURO
Plasmid#205466PurposeA backbone containing a RsDddA fragment D for constructing mitochondria-targeted RsDdCBE expression plasmidDepositorInsertMTS; N136; ccdb; RsDddA-D; UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP
Plasmid#204729PurposeCre-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP
Plasmid#204727PurposeFrt-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP
Plasmid#204724PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
iE61 PB-Zim3-XTEN-dCas9-mScarlet-puro-BFP
Plasmid#204722PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EFalpha-SVA-lncRNA-AK057321-mcherry-puro
Plasmid#202800PurposeLentiviral vector to express SVA-lncRNA AK057321 with ires for mcherry and puro expressionDepositorInsertSVA-lncRNA AK057321
UseLentiviralTagsExpressionMammalianMutationPromoterEFalpha (with intron)Available sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX2-Q6-R18-G1333C-UGI-Puro
Plasmid#198845PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cellsDepositorInsertCOX2-Q6-R18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ATP8-W9-R15-G1333N-UGI-Puro
Plasmid#198841PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertATP8-W9-R15 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ATP8-W9-L17-G1333C-UGI-Puro
Plasmid#198840PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cellsDepositorInsertATP8-W9-L17 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro
Plasmid#198844PurposeEmploy DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cellsDepositorInsertCOX2-Q6-L18 TALEN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NLS-ccdb-DddA(G1333C)-UGI-Puro
Plasmid#173232PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NLS-ccdb-DddA(G1333N)-UGI-Puro
Plasmid#173233PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NLS-ccdb-DddA(G1397C)-UGI-Puro
Plasmid#173234PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NLS-ccdb-DddA(G1397N)-UGI-Puro
Plasmid#173235PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ccdb-DddA(G1397C)-UGI-Puro
Plasmid#173230PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ccdb-DddA(G1397N)-UGI-Puro
Plasmid#173231PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV puro PRDM14 ΔSET+ΔZinc finger domains
Plasmid#193678PurposeConstitutive retroviral expression of PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseRetroviralTagsExpressionMutationDeletion of SET and Zinc finger domainsPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPbaCas13b-EGFP-NLS
Plasmid#191365PurposeOverexpression of NLS-dPbaCas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPbaCas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba5Cas13b-EGFP-NLS
Plasmid#191364PurposeOverexpression of NLS-dPba5Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba5Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba4Cas13b-EGFP-NLS
Plasmid#191363PurposeOverexpression of NLS-dPba4Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba4Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dHgm6Cas13b-EGFP-NLS
Plasmid#191366PurposeOverexpression of NLS-dHgm6Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dHgm6Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dBba2Cas13b-EGFP-NLS
Plasmid#191362PurposeOverexpression of NLS-dBba2Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dBba2Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba3Cas13b-EGFP-NLS
Plasmid#191361PurposeOverexpression of NLS-dPba3Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba3Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only