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pWZL Neo Myr Flag NME7

Plasmid

#20509

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

NME7 (NME7 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag ADCK4

Plasmid

#20544

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ADCK4 (ADCK4 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PAPSS1

Plasmid

#20557

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PAPSS1 (PAPSS1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCDH-V1-rCD20-BSD

Plasmid

#209752

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 1) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V2-rCD20-BSD

Plasmid

#209753

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V3-rCD20-BSD

Plasmid

#209754

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL delta PBM

Plasmid

#202415

Purpose

Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2)

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Lacking DTHL

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL FBM

Plasmid

#202417

Purpose

Expression of GFP-tagged PODXL FERM-binding motif mtuation (FBM; doesn't bind Ezrin)

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

H477/R479/S481>AAA mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL delta PBM + Ezrin

Plasmid

#202418

Purpose

Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) fused to EZRIN

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

Ezrin and GFP

Expression

Mutation

delete PDZ binding motif

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pE-N1-RDH11

Plasmid

#161916

Purpose

Expresses untagged human RDH11. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

Retinol dehydrogenase 11 (RDH11 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

Plasmid

#161924

Purpose

To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

sgRNA targeting RDH11 exon 2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Y478C-WLS-HA-IRES-GFP

Plasmid

#178063

Purpose

Expresssion of WLS-HA protein (Y478C mutant)

Depositor

Joseph Gleeson

Article

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Use

Tags

HA and IRES-GFP

Expression

Mammalian

Mutation

Y478C

Promoter

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Y392C-WLS-HA-IRES-GFP

Plasmid

#178062

Purpose

Expresssion of WLS-HA protein (Y392C mutant)

Depositor

Joseph Gleeson

Article

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Use

Tags

HA and IRES-GFP

Expression

Mammalian

Mutation

Y392C

Promoter

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-I531T-WLS-HA-IRES-GFP

Plasmid

#178064

Purpose

Expresssion of WLS-HA protein (I531T mutant)

Depositor

Joseph Gleeson

Article

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Use

Tags

HA and IRES-GFP

Expression

Mammalian

Mutation

I531T

Promoter

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag GK2

Plasmid

#20494

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

GK2 (GK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag VRK3

Plasmid

#20664

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

VRK3 (VRK3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-HSD17B11-WT-FLAGC

Plasmid

#161903

Purpose

Expresses human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

hydroxysteroid 17-beta dehydrogenase 11 (HSD17B11 Human)

Use

Tags

FLAG-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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rAAV9-CAP-in-cis-Lox-AAV

Plasmid

#205990

Purpose

rAAV9 cap acceptor vector for CREATE libraries

Depositor

Viviana Gradinaru

Article

Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

Insert

rAAV9 cap acceptor vector for CREATE libraries

Use

AAV

Tags

Expression

Mutation

Deletion between XbaI and AgeI sites

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-HSD17B13-WT-FLAGC

Plasmid

#184501

Purpose

Expresses human HSD17B13 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

17-beta-hydroxysteroid dehydrogenase 13 (HSD17B13 Human)

Use

Tags

FLAGC-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-BRAF-V600E

Plasmid

#15269

Purpose

Retroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

BRAF (BRAF Human)

Use

Retroviral

Tags

His and Myc

Expression

Mammalian

Mutation

Valine 600 to Glutamate (mutation has been found …

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge