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pC1-mitoRexYFP

Plasmid

#60246

Purpose

Mitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

Depositor

Vsevolod Belousov

Article

Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

Insert

mitoRexYFP

Use

Tags

Mitochondrial targeting signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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opto-Cdc42

Plasmid

#174510

Purpose

Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

Insert

opto-Cdc42-mCherry

Use

Tags

3xFLAG and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1319

Plasmid

#143490

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

KLF13 (KLF13 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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ptf-Galectin3

Plasmid

#64149

Purpose

Expression in mammalian cells

Depositor

Tamotsu Yoshimori

Article

Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

Insert

Galectin3 (LGALS3 Human)

Use

Tags

EGFP and mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCH67 (crRNA expression vector)

Plasmid

#217337

Purpose

CROP-seq crRNA expression vector with GFP and puro marker

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

AsDR-filler-AsDR8, GFP-puro

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

Plasmid

#209080

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

Depositor

Vikram Khurana

Article

Lam et al bioRxiv 2022.11.08.515615

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Use

Tags

sfGFP

Expression

Mammalian

Mutation

A53T

Promoter

TRE4G

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MKK1K97A

Plasmid

#107974

Purpose

expression of a dominant negative MAP Kinase Kinase

Depositor

Edwin Krebs

Article

Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7

Insert

MKK1 K97A (MAP2K1 Human)

Use

Tags

Expression

Mammalian

Mutation

dominant negative; see Depositor Comments below

Promoter

CMV

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

Plasmid

#196702

Purpose

Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 wild type

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pInducer-TAZ-S89A/S311A

Plasmid

#213589

Purpose

Doxycycline inducible expression of TAZ-S89A/S311A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

2xHA

Expression

Mammalian

Mutation

S89A, S311A

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

Plasmid

#113888

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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