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Plasmid#181850PurposemCherry-tagged DnaJB1-PKA catalytic subunit fusion; for mammalian expression.DepositorUseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1-N-Myc_1-205 USP7
Plasmid#131245PurposeMammalian expression of the USP7 TRAF-like domain with an N-terminal Myc tag.DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationExpresses a mutant form of our pcDNA3.1-N-Myc_1-5…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 S D614
Plasmid#158074PurposeExpresses SARS-CoV-2 spike proteinDepositorInsertSARS-CoV-2 spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/TagRFP-LL5b
Plasmid#112828Purposetransient/stable overexpressionDepositorInsertLL5b (Phldb2 Mouse)
UseTagsTagRFPExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Strep-Lrrk2-Myc
Plasmid#161582Purposeexpression of full-length mouse Lrrk2 with C-terminal Myc tagDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationnonePromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ eiF4A2 myc HIS
Plasmid#71658Purposemammalian expression of eiF4A2DepositorInsertEIF4A2 (EIF4A2 Human)
UseTagsHIS and mycExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ GEMIN6 myc HIS
Plasmid#71659PurposeMammalian expression of GEMIN6DepositorInsertGEMIN6 (GEMIN6 Human)
UseTagsHIS and mycExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-) MID51 4xMyc Hisx6
Plasmid#44598DepositorInsertMiD51 (Mief1 Mouse)
UseTags4xMyc and 6xHisExpressionMammalianMutationPromoterAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (WT)
Plasmid#73963PurposeSTAG2 cDNA (CCDS43990) wild typeDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationwild typePromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/myc-his Ndel1
Plasmid#12572DepositorInsertNdel1 (NDEL1 Human)
UseTagsHis and MycExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-JEDI-2P-Kv
Plasmid#179462PurposeSoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)DepositorInsertJEDI-2P-Kv
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-KB-1753-Nluc
Plasmid#158603PurposePlasmid expressing KB-1753-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Clover-Prx2-mRuby2
Plasmid#117906Purposefluorescent reporter of cytosolic hydrogen peroxideDepositorInsertClover-Prx2-mRuby2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CCND1
Plasmid#172648PurposeExpresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cellsDepositorInsertCCND1 (CCND1 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD9-(TEV)-tTA
Plasmid#162596Purposehuman CD9-TEV cleavage site-tTADepositorInserthuman CD9 (CD9 Human)
UseTagsTEV protease cleavage site and tTAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hAsCpf1(TYCV) (pY210)
Plasmid#89351PurposeExpresses humanized AsCpf1 variant that recognizes TYCV PAMsDepositorInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K607RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-ASAP2s
Plasmid#101274PurposeGenetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)DepositorInsertASAP2s
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS-CoV-2-S (D614G)
Plasmid#215838PurposeExpresses SARS-CoV-2 Spike protein in mammalian cellsDepositorInsertSARS-CoV-2 Spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CDK6
Plasmid#172614PurposeExpresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cellsDepositorInsertCDK6 (CDK6 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TAT-1-101-FLAG
Plasmid#35981DepositorInserttransactivating protein (tat )
UseTagsFlagExpressionMammalianMutationtat from HIV1 101 amino acidsPromoterAvailable sinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only