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pcDNA3.1-CHRFAM7A-mCherry

Plasmid

#62635

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

Expression

Mammalian

Mutation

mCherry inserted into M3-M4 loop

Promoter

CMV

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pCDH_SBP-mCherry-GPI

Plasmid

#65299

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Lentiviral

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-Rab11

Plasmid

#174461

Purpose

Express mCherry with recycling endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with recycling endosome-targeting sequence

Use

Tags

small GTPase (Rab11)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMAH2-intein/mCherry

Plasmid

#71405

Purpose

Expresses suppression tRNA and mCherry inserted with a Npu DnaE intein

Depositor

Huiwang Ai

Article

Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8

Insert

Npu DnaE Intein insertion in mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only
ANO6-Plvx-mCherry-c1

Plasmid

#62554

Purpose

Full length mouse ANO6 tagged with mCherry on the C-terminus

Depositor

Renzhi Han

Article

Zhao et al PLoS One. 2014 Mar 24;9(3):e92749. doi:

Insert

mouse ANO6 (Ano6 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

CMV

Available since

March 3, 2015

Availability

Academic Institutions and Nonprofits only

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DD-DamWT-IRES2-mCherry

Plasmid

#159602

Purpose

Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the wild-type allele of Dam.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamWT-IRES2-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-PI(WT)-Vav2(DPZ)

Plasmid

#86974

Purpose

PI(DM): Photo-inhibitable/light sensitive; DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/C1 (Vav2 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-ATG5-K130R

Plasmid

#13096

Depositor

Roberta Gottlieb

Article

Hamacher-Brady et al Cell Death Differ. 2006 Apr 28. ():.

Insert

ATG5-K130R (Atg5 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

K130R

Promoter

Available since

Nov. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_mCherry_mito Grx1-roGFP2

Plasmid

#155044

Purpose

Fluorescent redox reporter

Depositor

Vanessa LaPointe

Insert

Glutaredoxin-1 (GLRX Human)

Use

Lentiviral

Tags

mitochondrial targeting sequence and roGFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pICE-NLS-mCherry

Plasmid

#60364

Purpose

Plasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.

Depositor

Patrick Calsou, Sébastien Britton

Article

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Insert

NLS-mCherry

Use

Tags

NLS from SV40 T antigen and mCherry

Expression

Mutation

Promoter

CMV-tet

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-FKBP:mCherry-Rab5a

Plasmid

#72901

Purpose

Early endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab5a (RAB5A Human)

Use

Lentiviral

Tags

FKBP(F36V) and mCherry

Expression

Mammalian

Mutation

fragment a.a. 2-215

Promoter

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-GOLGA4-GRIP

Plasmid

#220068

Purpose

overexpreeses the TGN protein GRIP with a mCherry fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 22;7:2

Insert

GOLGA4-GRIP (GOLGA4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMK281 (mCherry2-Hygro)

Plasmid

#72797

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Hygro

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA5_FRT_TO_TurboID-mCherry-Cry2

Plasmid

#166504

Purpose

Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.

Depositor

Angelos Constantinou

Article

Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2

Insert

None

Use

Tags

TurboID and mCherry-CRY2

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pME-mCherry-24xMBS

Plasmid

#86242

Purpose

Middle entry vector for generating MBS tagged RNAs with a fluorescent protein reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

mCherry-24xMBS

Expression

Mutation

Promoter

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLoxP-DHFR-mCherry

Plasmid

#70147

Purpose

Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion

Depositor

David Sibley

Article

Long et al Infect Immun. 2016 Jan 11. doi: 10.1128/

Insert

a selectable dihydrofolate reductase-thymidylate synthase marker

Use

Expression in toxoplasma gondii

Tags

mCherry

Expression

Mutation

Promoter

DHFR 5' UTR

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-p62-Silent_Mutation_WT

Plasmid

#187986

Purpose

human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

silent mutation for resistance to sip62 #5

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pIRES Centrin1 mCherry

Plasmid

#64338

Purpose

Bicistronic expression of mCherry-Centrin1 fusion and Hygro resistance gene

Depositor

Matthieu Piel

Insert

Centrin1

Use

Tags

IRES-Hygro and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pUltraHot-mCherry-BLM-WT

Plasmid

#206966

Purpose

expressing the protein BLM fused to mCherry in human cells

Depositor

Eran Hornstein

Article

Danino et al Nucleic Acids Res. 2023 Jul 28:gkad613.

Insert

Bloom Syndrome RecQ Like Helicase (BLM Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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p2K7bsdUBI-mCherry-STIM1

Plasmid

#114178

Purpose

Lentiviral vector for expression of mCherry-STIM1

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

STIM1 (STIM1 Human)

Use

Lentiviral

Tags

mCherry (inserted after signal peptide)

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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