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pcDNA3.1-p300

Plasmid

#23252

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

p300 (EP300 Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2010

Availability

Academic Institutions and Nonprofits only

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pT2/LTR7-GFP

Plasmid

#62541

Purpose

LTR7-driving reporter

Depositor

Zsuzsanna Izsvak

Article

Wang et al Nature. 2014 Dec 18;516(7531):405-9. doi

Insert

LTR7-GFP

Use

Sleeping beauty transposon

Tags

Expression

Mammalian

Mutation

Promoter

LTR7

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti_ABE8e-SpRY-P2A-BFP_HygroR

Plasmid

#213009

Purpose

A lentiviral vector used to create stable cell lines expressing the ABE8e-SpRY base editor and BFP

Depositor

Richard Sherwood

Article

Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

Insert

ABE8e-SpRY-D10A

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A nickase variant of SpRY

Promoter

Ef-1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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NFAT/AP-1 3x luciferase

Plasmid

#11783

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

NFAT/AP-1 3x (Il2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pREXC3GH

Plasmid

#47077

Purpose

FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Lactococcus lactis expression

Tags

3C protease site, GFP, and His

Expression

Mutation

Promoter

NIS (nisin)

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag ERbeta

Plasmid

#35562

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

Insert

Estrogen Receptor beta (ESR2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2012

Availability

Academic Institutions and Nonprofits only

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CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro

Plasmid

#83306

Purpose

Lentivirus vector to express guideRNA and dCas9 with puro resistant gene

Depositor

Tohru Kimura

Article

Murakami et al Nat Immunol. 2021 Mar;22(3):301-311. doi

Insert

sgRNA and dCas9 from pX330

Use

Lentiviral

Tags

FLAG and PA tags

Expression

Mammalian

Mutation

Promoter

U6 for sgRNA and CBh for dCas9

Available since

Dec. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCGN N-Ras wt

Plasmid

#14723

Depositor

Adrienne Cox

Article

Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

Insert

N-ras (NRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2007

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec16A

Plasmid

#36155

Purpose

Expresses Sec16A as a GFP fusion

Depositor

David Stephens

Article

Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

Insert

Sec16A (SEC16A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCXLE-mp53DD

Plasmid

#41859

Purpose

Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

Trp 53 (Trp53 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Δ40-903

Promoter

CAG

Available since

July 17, 2013

Availability

Academic Institutions and Nonprofits only

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rAAV2-retro helper

Plasmid

#81070

Purpose

retrograde AAV helper vector, carrying rep and modified capsid gene

Depositor

David Schaffer, Alla Karpova

Article

Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

Inserts

rep
rAAV2-retro cap

Use

AAV and Unspecified; Helper vector

Tags

Expression

Mutation

Pro2Ala, possibly Asp613Gly and changes made to A…

Promoter

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-H-Ras_wt

Plasmid

#39503

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS (HRAS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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FLAG-MATR3

Plasmid

#32880

Depositor

Yossi Shiloh

Article

Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

Insert

MATR3 Matrin3 (MATR3 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pOTTC407 - pAAV EF1a eGFP

Plasmid

#60058

Purpose

An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

enhanced Green Fluorescent Protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-FHC-POLQ

Plasmid

#64875

Purpose

lentiviral expression of human POLQ

Depositor

Richard Wood

Article

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pRDA_550

Plasmid

#203398

Purpose

All-in-one lentiviral expression of gRNA and EnAsCas12a

Depositor

John Doench

Article

Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

Insert

Enhanced Acidaminococcus Cas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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Degron-KI donor backbone

Plasmid

#65483

Purpose

Donor backbone vector to clone left and right HR arms in for Degron-KI application

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pSEM318 - sgRNA mosTI unc-119 single-copy

Plasmid

#159822

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 single-copy

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Blast DEST ERKKTRmRuby2

Plasmid

#90231

Purpose

Lentiviral vector to express ERK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

ERK Kinase Translocation Reporter

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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LifeAct-mGarnet2

Plasmid

#104308

Purpose

codes for a fusion of fluorescent protein mGarnet2 and lifeact that targets F-actin in live cells

Depositor

Ulrich Nienhaus

Article

Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

Insert

LifeAct-mGarnet2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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