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Showing: 1281 - 1300 of 18490 results

  1. pCDH-CMV-mCherry-T2A-Puro

    Plasmid
    #72264
    Purpose
    Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.
    Depositor
    Kazuhiro Oka
    Insert
    mCherry-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72264-plasmid-map-sequence-id-322454

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  5. PB-TO-hNGN2

    Plasmid
    #172115
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172115-plasmid-map-sequence-id-448148

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  11. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Use
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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  12. pRRLSIN.cPPT.PGK-GFP.WPRE

    Plasmid
    #12252
    Purpose
    3rd generation lentiviral backbone
    Depositor
    Didier Trono
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2007
    Availability
    Academic Institutions and Nonprofits only
    12252-plasmid-map-sequence-id-444115

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  14. tet-pLKO-sgRNA-puro

    Plasmid
    #104321
    Purpose
    3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.
    Depositor
    Nathanael Gray
    Article
    Huang et al Elife. 2017 Sep 19;6. pii: e26693. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    104321-plasmid-map-sequence-id-323839

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  19. pET-MCN-10His-mStayGold (E138D)

    Plasmid
    #211361
    Purpose
    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.
    Depositor
    Mohan Balasubramanian
    Article
    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103
    Insert
    mStayGold
    Use
    Tags
    10xHis-tag
    Expression
    Bacterial
    Mutation
    E138D
    Promoter
    T7
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    211361-plasmid-map-sequence-id-417678

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Showing: 1281 - 1300 of 18490 results