Skip to main content
Addgene
Showing: 6421 - 6430 of 8651 results

  1. HTR5-DuET

    Plasmid
    #213325
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR5 (HTR5A Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213325-plasmid-map-sequence-id-430860

    Enlarge

  2. HTR7-DuET

    Plasmid
    #213327
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR7 (HTR7 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213327-plasmid-map-sequence-id-430861

    Enlarge

  3. HTR2B-DuET

    Plasmid
    #213322
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR2B (HTR2B Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213322-plasmid-map-sequence-id-430858

    Enlarge

  4. HTR1A-DuET

    Plasmid
    #213316
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR1A (HTR1A Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213316-plasmid-map-sequence-id-430853

    Enlarge

  5. F2RL3-DuET

    Plasmid
    #213237
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    F2RL3 (F2RL3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213237-plasmid-map-sequence-id-430795

    Enlarge

  6. F2RL1-DuET

    Plasmid
    #213235
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    F2RL1 (F2RL1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213235-plasmid-map-sequence-id-430793

    Enlarge

  7. pFSynW SYT9 D197, 199, 330, 332N IRES GFP

    Plasmid
    #195703
    Purpose
    Lentiviral plasmid encoding SYT9 with D197, 199, 330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter
    Depositor
    Edwin Chapman
    Article
    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.
    Insert
    Syt9 (Syt5 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    full-length mouse SYT9 with D197, 199, 330, 332N …
    Promoter
    Available since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195703-plasmid-map-sequence-id-383663

    Enlarge

  9. pKTop::sptse5-CT (1169-1229)

    Plasmid
    #192948
    Purpose
    For membrane insertion study; expresses Tse5-CT (1169-1229)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT (1169-1229)
    Use
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for tse5 (1169-1229)
    Promoter
    Available since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192948-plasmid-map-sequence-id-380616

    Enlarge

Showing: 6421 - 6430 of 8651 results