pUT1888-HQ858801
(Plasmid
#27883)
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27883 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR/SD
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2601
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Vector typeEntry vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsE. coli TOP10
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameORPHAN transcription factor from EB707293
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SpeciesZea mays
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Insert Size (bp)792
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GenBank IDHQ858801
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 For
- 3′ sequencing primer M13 Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This CDS was amplified using PCR from a full length cDNA template from the Maize Full-length cDNA Project (www.maizecdna.org) and the PCR product included the stop codon. The PCR product was cloned into a Gateway pENTR/SD vector.
Further information on this gene may be obtained at www.grassius.org using the GenBank number. (The second half of the plasmid name is the GenBank Number for the sequence of the insert)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUT1888-HQ858801 was a gift from John Gray & Erich Grotewold (Addgene plasmid # 27883 ; http://n2t.net/addgene:27883 ; RRID:Addgene_27883) -
For your References section:
The Maize TFome--development of a transcription factor open reading frame collection for functional genomics. Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E. Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 Aug 26. 10.1111/tpj.12623 PubMed 25053252