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Opto-ACTU−

Plasmid

#198869

Purpose

Optogenetic recruitment construct to increase inner membrane surface charge

Depositor

Peter Devreotes

Article

Banerjee et al Nat Cell Biol. 2022 Oct;24(10):1499-1515

Insert

Rad17 polyanionic tail (-14)

Use

Synthetic Biology

Tags

CRY2PHR-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(CAT)T7-SB100

Plasmid

#34879

Depositor

Zsuzsanna Izsvak

Article

Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

Insert

SB100X transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pMY-empty-MCS

Plasmid

#163351

Purpose

The empty pMY backbone with multiple cloning site

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTYF-sGFAP-LOx-IRES-tdTomato

Plasmid

#166919

Purpose

Express the bacterial lactate oxidase (LOx) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Insert

Lactate oxidase

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

sGFAP

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-IRES-eGFP

Plasmid

#163361

Purpose

Retroviral backbone for in-frame cloning of a gene of interest and an IRES separated GFP marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-MCS-P2A-eGFP

Plasmid

#163356

Purpose

Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated GFP marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-EFS-MCS-WPRE

Plasmid

#163362

Purpose

The empty pLenti backbone with multiple cloning site that allows shuttling between pMY and pMX vectors

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-mCherry-P2A-PuroR-HA

Plasmid

#163342

Purpose

Retroviral vector to express cytosolic mCherry and an HA-tagged puromycin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

mCherry

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-MCS-P2A-Ly6G

Plasmid

#163353

Purpose

Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Ly6G surface marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-eGFP-P2A-PuroR-HA

Plasmid

#163343

Purpose

Retroviral vector to express cytosolic GFP and an HA-tagged puromycin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-MCS-P2A-BlastR-DYK

Plasmid

#163357

Purpose

Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Blasticidin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMY-MCS-P2A-PuroR-HA

Plasmid

#163352

Purpose

Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Puromycin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-MCS-P2A-CD90.1

Plasmid

#163354

Purpose

Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.1 surface marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-MCS-GSG-eGFP

Plasmid

#163350

Purpose

Retroviral backbone for in-frame cloning of a C-terminally GFP labeled gene of interest.

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMY-MCS-P2A-CD90.2

Plasmid

#163355

Purpose

Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.2 surface marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-mTurquoise2-P2A-PuroR-HA

Plasmid

#163344

Purpose

Retroviral vector to express cytosolic mTurquoise2 and an HA-tagged puromycin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

mTurquoise2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-mVenus-P2A-PuroR-HA

Plasmid

#163345

Purpose

Retroviral vector to express cytosolic mVenus and an HA-tagged puromycin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

mVenus

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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phDLX2-N174

Plasmid

#60860

Purpose

2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

DLX2 (DLX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_SIK3_CR_T221E

Plasmid

#108108

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

change guanine 501 to cytosine (silent mutation),…

Promoter

EFS promoter

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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LV-hGFAP-DLX2-ires-GFP

Plasmid

#213602

Purpose

Expression of human DLX2 and GFP under the human GFAP ABC1D promoter

Depositor

Chun-Li Zhang

Article

Zhang et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

DLX2 (DLX2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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