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Plasmid#158119Purposedeletion mADAR2DepositorInsertAdar2 (Adarb1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmirGlo-RBM8A-3'UTRAluY-Sz mut 2598-4066
Plasmid#171135PurposereporterDepositorInsertRBM8A 3'UTR (RBM8A Human)
UseTagsExpressionMammalianMutationA3302G, A3356G, A3373G, A3389G,A3451G, T3729C, A3…PromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode
Plasmid#170236PurposeBarcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCHUK (CHUK Human)
UseLentiviralTagsV5ExpressionMutationS176E, S180EPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET3d-SF-DCTN1-TS
Plasmid#169375PurposeE. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.DepositorInsertdynactin subunit 1 isoform 1 (DCTN1 Human)
UseTags6xHis Tag, SplitFlAsH Tag, and TwinStrep TagExpressionBacterialMutationPoint mutations: A139T, I304T, Q322R, T516A, E577…PromoterAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
BPK1520 sgCHMP1A
Plasmid#169321PurposeExpresses CHMP1a targeting sgRNA in non-lentiviral BPK1520 plasmidDepositorInsertsgCHMP1A
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
BPK1520 sgCHMP4B
Plasmid#169322PurposeExpresses CHMP4B targeting sgRNA in non-lentiviral BPK1520 plasmidDepositorInsertsgCHMP4B
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
RG6-BAP1_E11
Plasmid#154025PurposeBichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 11DepositorInsertBAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)
UseSplicing reporterTagsDsRed1, FLAG, SV40 NLS, and eGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
RG6-BAP1_E7
Plasmid#154023PurposeBichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 7DepositorInsertBAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)
UseSplicing reporterTagsDsRed1, FLAG, SV40 NLS, and eGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hMLH1dn
Plasmid#174828PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)DepositorInsertPEmax-P2A-hMLH1dn
UseTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-mVenus-p27K−
Plasmid#176651PurposeIt encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).DepositorInsertmVenus-p27K- (Cdkn1b Mouse)
UseLentiviralTagsmVenusExpressionMutationMutant K- (lacks affinity to Cdk)PromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-SNAP-hCB2_IRES_EGFP
Plasmid#223506PurposeExpression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection markerDepositorInsertSNAP-tag-CNR2 and EGFP (CNR2 Synthetic, Human)
UseTagsSNAP-tagExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-BSD
Plasmid#174821PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
UseTagsP2A-BSD, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tet-off TauRD (P301L/V337M)
Plasmid#188572PurposeDox-repressible expression of the Tau repeat domainDepositorInsertTau repeat domain (MAPT Human)
UseLentiviralTagsMycExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-hMLH1dn
Plasmid#174827PurposeMammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)DepositorInsertPE2-P2A-hMLH1dn
UseTagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-Nm-dCas9-p300 Core
Plasmid#61365Purposeencodes N. meningiditis dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertNm-dCas9-p300 Core (EP300 Synthetic, Human, Neisseria meningiditis)
UseCRISPR and Synthetic BiologyTagsHA tag and NLSExpressionMammalianMutationD16A, D587A, H588A, N611A in Nm Cas9PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-FLp300
Plasmid#61356Purposeencodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-HAPLN1
Plasmid#200777PurposeExpresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoterDepositorInsertHyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
UseAAVTagscfGFPExpressionMutationPromoterSynapsinAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-∆Hapln1
Plasmid#200778PurposeExpresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoterDepositorInsertHyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
UseAAVTagscfGFPExpressionMutationdeleted lectican binding domain (aminoacids 40-15…PromoterSynapsinAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.0-Magneto2.0-p2A-mCherry
Plasmid#74308PurposeBicistronic expression of Magneto2.0 and mCherry in mammalian cellsDepositorUseTagsFlag and p2A-mCherryExpressionMammalianMutationTRPV4: delta 760–871PromoterCMVAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR 223 VPS4B
Plasmid#169320PurposeGateway entry vector for human VPS4B ORFDepositorInsertVPS4B (VPS4B Human)
UseGateway entry vectorTagsExpressionMutationPromoterNAAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only