-
Plasmid#79469PurposesgRNA shuttle vector; module 5DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE13
Plasmid#79471PurposesgRNA shuttle vector; module 6DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE14
Plasmid#79473PurposesgRNA shuttle vector; module 7DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE15
Plasmid#79474PurposesgRNA shuttle vector; module 8EDepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE10
Plasmid#79466PurposesgRNA shuttle vector; module 4DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239A (dFnCas12a)-TV
Plasmid#136379PurposeCRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation systemDepositorInsertdFnCas12a-TV
UseCRISPRTagsExpressionPlantMutationD917APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaBC (pCLK1210)
Plasmid#50448PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylaseDepositorInsertPPCS PPCDC
UseTags6XHIS tagExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0503
Plasmid#91013PurposeModule A, Promoter: CmYLCV, Gene: Csy4-P2A-AtCas9, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9
UseCRISPRTagsExpressionMutationPromoterCmYLCVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3013
Plasmid#91100PurposeModule C, Promoter: PvUbi2, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoterPvUbi2Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3501
Plasmid#91050PurposeModule A, Promoter: 35S, Gene: dAtCas9-VPR, Terminator: HSPDepositorInsertdAtCas9-VPR
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A4110
Plasmid#91056PurposeModule A, Promoter: ZmUbi, Gene: Csy4-P2A-dTaCas9-VPR, Terminator: HSPDepositorInsertCsy4-P2A-dTaCas9-VPR
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCk1
Plasmid#136695PurposeAcceptor plasmid for odd level position 1, lacZ blue-white screening. Part of the OpenPlant toolkit.DepositorInsertlacZ
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_omega2
Plasmid#68239PurposeGoldenBraid level 1 Omega plant expression vectorDepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
HGXPRT
Plasmid#64201PurposeExpression of Plasmodium falciparum HGXPRTDepositorInsertHypoxanthine guanine xanthine phosphoribosyltransferase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 SV40-AcrIIA4 (GB3333)
Plasmid#160605PurposeSV40-AcrIIA4 codon optimized for N.benthamianaDepositorInsertAcrIIA4
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
P16ΔS:S10-(MCS)-3xHA
Plasmid#108183PurposeBinary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterP16ΔSAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBRC169
Plasmid#79670PurposeDsREDmonomer-based BiFC vector for transient expression of RC (169-225) in plants.DepositorTypeEmpty backboneUseReporter plasmidTagsDsRED-monomer (169–225)/RC169ExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE5
Plasmid#79461PurposesgRNA shuttle vector; module 1DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19/CF-mChe
Plasmid#183166PurposeConstruct for transient expression of cytoplasmic foci-targeted mCherry in plants.DepositorInsertCF-mCherry
UseTagsAtTZF1, A. thaliana TANDEM ZINC FINGER PROTEIN 1ExpressionPlantMutationPromoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Tp-mChe
Plasmid#183165PurposeConstruct for transient expression of tonoplast-targeted mCherry in plants.DepositorInsertTp-mCherry
UseTags28 aa of A. thaliana CALCINEURIN B-LIKE PROTEIN 2…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLIPglu-3.2mDelta13
Plasmid#12992DepositorInsertFLIPglu F16A S112A Delta13
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationF16A, S112A in binding proteinPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFLIPglu-170nDelta13
Plasmid#12989DepositorInsertFLIPglu F16F Delta13
UsePlant nanosensorTagsECFP and EYFPExpressionPlantMutationWT binding protein (no F16A)PromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_omega1
Plasmid#68238PurposeGoldenBraid level 1 Omega plant expression vectorDepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2
Plasmid#68229PurposeGoldenBraid level 1 Alpha plant expression vector.DepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJA053
Plasmid#128515PurposeExpresses SFA8 in PichiaDepositorInsertSFA8 a 2S albumin from sunflower (LOC110892047 Helianthus annus)
UseTagsExpressionYeastMutationPromoterAOX1Available sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE7
Plasmid#79462PurposesgRNA shuttle vector; module 2DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE9
Plasmid#79465PurposesgRNA shuttle vector; module 3DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE11
Plasmid#79467PurposesgRNA shuttle vector; module 4EDepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
P16ΔS:(MCS)-3xHA-S11
Plasmid#108185PurposeBinary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterP16ΔSAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3910
Plasmid#91054PurposeModule A, Promoter: ZmUbi, Gene: dTaCas9-VPR, Terminator: HSPDepositorInsertdTaCas9-VPR
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291
Plasmid#129671PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationBthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C4300
Plasmid#91103PurposeModule C, Promoter: none, Gene: BaeI LacZ cassette for GT donor cloning, Terminator: noneDepositorInsertBaeI LacZ cassette for GT donor cloning
UseUnspecifiedTagsExpressionMutationPromoternoneAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBCN155-MXMT
Plasmid#79815PurposeCFP-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsCN155/CFP (1–155)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ293 (BhCas12b_v4)
Plasmid#136383PurposeGateway entry clone for BhCas12b_v4DepositorInsertBhCas12b_v4
UseCRISPRTagsExpressionPlantMutationK846R/S893R/E837GPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3601
Plasmid#91051PurposeModule A, Promoter: 35S, Gene: dAtCas9-VP64, Terminator: HSPDepositorInsertdAtCas9-VP64
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-PAT-ProGL2 GW
Plasmid#80689Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterProGL2Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1R
Plasmid#68230PurposeGoldenBraid level 1 Alpha plant expression vector.DepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2R
Plasmid#68231PurposeGoldenBraid level 1 Alpha plant expression vector.DepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GB4080
Plasmid#215249PurposeModule for the constitutive expression of EGFP, p19 and the N. nambi luminescence pathway genes fused to a SF.DepositorInsertSF-EGFP-p19-LUZ-H3H-HISPS-CPH
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4003
Plasmid#215248PurposeModule for the constitutive expression of PIF6 and PhyB:VP16DepositorInsertPIF6-PhyB:VP16
UseSynthetic BiologyTagsExpressionMutationV234L in PIF6- please see dep. sequence, BsaI and…PromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
Jonah-1
Plasmid#228899PurposeJonah-1 localizes to the outer cyst wall of Acanthamoeba tagged with eGFPDepositorInsertJonah-1
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwn promoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Luke-2
Plasmid#228900PurposeLuke-2 localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFPDepositorInsertCarbohydrate binding domain cbm49 protein
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwnAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Leo-A
Plasmid#228901PurposeLeo-A localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFPDepositorInsertLeo-A
UseAcanthamoeba expression vectorTagseGFPExpressionMutationnonePromoterOwnAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4653
Plasmid#215267PurposeTU for the constitutive expression of the flippase (FLP) recombinase with a nopaline synthase (Nos) promoterDepositorInsertpNos:FLP:tNos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4829
Plasmid#215275PurposeTU for the constitutive expression of the N. nambi NPGA gene under the P35S promoter and the TNOS terminatorDepositorInsertP35S:NPGA:TNOS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB3774
Plasmid#215247PurposeCDS of CPH from N. nambi codon optimized for N.benthamiana. This gene is the final step and recycling of the bioluminiscente pathwayDepositorInsertCPH
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4807
Plasmid#215273PurposeModule for the expressino of the N. nambi HISPS gene under the PNOS promoter and the constitutive expression of EGFP and p19.DepositorInsertPNOS:HISPS-SF-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4808
Plasmid#215274PurposeModule for the expressino of the N. nambi HISPS gene under the P35S promoter and the constitutive expression of EGFP and p19.DepositorInsertP35S:HISPS-SF-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zhou-pLOV-GFP-Luc
Plasmid#161030PurposeLentiviral vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection.DepositorInsertGFP and luciferase
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4832
Plasmid#215276PurposeModule for the constitutive expression of HISPS, H3H, EGFP and p23.DepositorInsertHISPS-H3H-EGFP-p23
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only