159,530 results

1. pCDNA3-STAT3-Y705F

   Plasmid

   #74434

   Purpose

   Expression of STAT3-Y705F

   Depositor

   Jie Chen

   Article

   Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

   Insert

   Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Mutations E16K, Y705F

   Promoter

   CMV

   Available since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

   Plasmid

   #180263

   Purpose

   Lentiviral expression vector for dCas9-VP64 in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-TLR3-CFP

   Plasmid

   #13641

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 3 (TLR3 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2+ GCamp6-3NLS

   Plasmid

   #165401

   Purpose

   Expresses a nuclear localized genetically encoded calcium reporter.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

   Insert

   Nuclear Localization Signal
   

   Use

   Avian, xenopus, zebrafish

   Tags

   GCamp6

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV_iPE-C_P2A_Csy4

   Plasmid

   #232428

   Purpose

   Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

   Insert

   iPE-C–P2A–Csy4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSBtet-Pur

   Plasmid

   #60507

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TCE & RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-myrlysin/BORCS5

   Plasmid

   #194332

   Purpose

   Expresses myrlysin in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Pu et al Dev Cell. 2015 Apr 20;33(2):176-88. doi:

   Insert

   BORCS5 (BORCS5 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCI-neo-(HA)3-ubiquitin

   Plasmid

   #196991

   Purpose

   Expresses HA-tagged ubiquitin in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

   Insert

   ubiquitin (UBC Human)
   

   Use

   Tags

   triple HA tag

   Expression

   Mammalian

   Mutation

   silent mutation in ubiquitin codon 13

   Promoter

   CMV

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKSE401

   Plasmid

   #62202

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   35S and AtU6-26p

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-CMV-LoxP-DsRed-LoxP-eGFP

    Plasmid

    #65726

    Purpose

    Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

    Depositor

    Jacco van Rheenen

    Article

    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

    Insert

    LoxP-DsRed-LoxP-eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGP-CMV-jGCaMP8s

    Plasmid

    #162371

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    CMV

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLX_305_Ovalbumin

    Plasmid

    #184924

    Purpose

    Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

    Depositor

    Arlene Sharpe

    Article

    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

    Insert

    Ovalbumin (OVAL Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Our vector has an A188T mutation that does not im…

    Promoter

    PGK

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 6xHis-SNAP-PI(4)P Probe

    Plasmid

    #211509

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    SidC (P4C)
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAV0286

    Plasmid

    #149333

    Purpose

    Bacterial expression of SENP_EuB protease

    Depositor

    Dirk Görlich

    Article

    Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

    Insert

    SENP_EuB protease
    

    Use

    Tags

    His14 and Tev protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCherry-VAPB

    Plasmid

    #108126

    Purpose

    ER marker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:VAPB (VAPB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-ATF6

    Plasmid

    #32955

    Purpose

    For visualization of GFP-ATF6 in mammalian cells.

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    M67L and M313I

    Promoter

    CMV

    Available since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLS-SceI

    Plasmid

    #137725

    Purpose

    Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

    Depositor

    Nadav Ahituv

    Article

    Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 APEX2-NES

    Plasmid

    #49386

    Purpose

    Expresses APEX2 in the cytosol of mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Flag - APEX2 - NES
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. P336-FBP_11

    Plasmid

    #139702

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiCRISPRv2 puro

    Plasmid

    #98290

    Purpose

    Variant of lentiCRISPRv2 that confers puromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge