pCI-neo-(HA)3-ubiquitin
(Plasmid #196991)

• Purpose: Expresses HA-tagged ubiquitin in mammalian cells
• Depositing Lab: Juan Bonifacino
• Publication: Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83. doi: 10.1074/jbc.M509939200. Epub 2006 Jan 5.

Backbone

• Vector backbone: pCI-neo
• Backbone size w/o insert (bp): 5472
• Total vector size (bp): 5788
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ubiquitin
• Species: H. sapiens (human)
• Insert Size (bp): 329
• Mutation: silent mutation in ubiquitin codon 13
• Entrez Gene: UBC (a.k.a. HMG20)
• Promoter: CMV
• Tag / Fusion Protein:
  • triple HA tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pCI-neo forward
• 3′ sequencing primer: pCI-neo reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

triple HA tag subcloned between XhoI and EcoRI sites of pCI-neo; ubiquitin subcloned between EcoRI and SalI sites of pCI-neo