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Plasmid#69599PurposeSnap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondriaDepositorInsertSnap-Omp25
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV mCherry-GFP-LC3B WT
Plasmid#123230PurposeExpresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pANAP
Plasmid#48696Purposeallows co-translational incorporation of a fluorescent AA directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
UseTagsExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
BPK2660
Plasmid#70709PurposeHuman expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3G-p300-dCas9-P2A-mCherry
Plasmid#138456Purpose2nd Generation Lentiviral vector. Expresses an N-terminal p300-dCas9 fusion protein and mCherryDepositorInsertp300 core
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-human MYCN-EF1-mRFP
Plasmid#193708PurposeLentiviral expression of human MYCNDepositorInsertMYCN (MYCN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FoxO1-Clover
Plasmid#67759PurposeFluorescent fusion protein for tracking Akt signalingDepositorInsertFox01 (Foxo1 Mouse)
UseLentiviralTagsCloverExpressionMutationChanged Serine 209 to Alanine, changed Histidine …PromoterEF1aAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
mito-gTEMP_pcDNA3
Plasmid#109117PurposeMitochondria targetting gTEMPDepositorInsertmito-gTEMP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM200
Plasmid#13079DepositorInsertPtac-gam-bet-exo operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
HP4_M13
Plasmid#120340PurposeHelper plasmid for production of phagemid ssDNA, based on m13mp18DepositorInsertCoding part of the M13 phage genome
UseTagsExpressionBacterialMutationPromoternative phage promotersAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only