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Showing: 2441 - 2480 of 149193 results

  2. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

    Plasmid
    #107581
    Purpose
    B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    mCherry
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pdh promoter Bacillus megaterium DSM319
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    107581-plasmid-map-sequence-id-209155

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  4. pGR420

    Plasmid
    #213782
    Purpose
    Integrates onto p1 a partial LEU2 sequence to be subsequently used as an integration flank
    Depositor
    Chang Liu
    Insert
    MET15
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213782-plasmid-map-sequence-id-424626

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  5. CMV-mt4xi-GCaMP6f

    Plasmid
    #192075
    Purpose
    Fluorescent reporter for mitochondrial calcium
    Depositor
    Timothy Ryan
    Insert
    4 repeats of improved COX8 signal peptide plus GCaMP6f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    192075-plasmid-map-sequence-id-378919

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  6. pcDNA3.1-HA-Bax

    Plasmid
    #191944
    Purpose
    Expression of HA-Bax.
    Depositor
    Luca Scorrano
    Insert
    HA-Bax (Bax Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    191944-plasmid-map-sequence-id-379568

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  8. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

    Plasmid
    #185918
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185918-plasmid-map-sequence-id-367359

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  9. TFORF0718

    Plasmid
    #141671
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TEF (TEF Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141671-plasmid-map-sequence-id-391482

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  14. AVPR1B-Tango

    Plasmid
    #66226
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    AVPR1B (AVPR1B Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pGAPZ-STE2-1D4

    Plasmid
    #133423
    Purpose
    Yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    C59S, C252A. No alpha factor secretion signal in …
    Promoter
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133423-plasmid-map-sequence-id-267651

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  17. pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

    Plasmid
    #197500
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197500-plasmid-map-sequence-id-386637

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  19. pBP-Pspac

    Plasmid
    #112778
    Purpose
    Promoter repressed by LacI (not natively produced in B. subtilis 168)
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    Pspac promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112778-plasmid-map-sequence-id-218361

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  21. pAAV-Sox2-CreERT2

    Plasmid
    #135618
    Purpose
    Donor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locus
    Depositor
    Lin He
    Article
    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e
    Insert
    P2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    P2A-CreERT2
    Expression
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135618-plasmid-map-sequence-id-292032

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  29. pGR481

    Plasmid
    #213783
    Purpose
    Integrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutations
    Depositor
    Chang Liu
    Insert
    ura3*
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213783-plasmid-map-sequence-id-424628

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  35. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139998-plasmid-map-sequence-id-394975

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  38. pLKO.3G

    Plasmid
    #14748
    Depositor
    Christophe Benoist, Diane Mathis
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 5, 2007
    Availability
    Academic Institutions and Nonprofits only
    14748-plasmid-map-sequence-id-352429

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  40. pSBbi-RP

    Plasmid
    #60513
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60513-plasmid-map-sequence-id-373029

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Showing: 2441 - 2480 of 149193 results